PMID: 8589073Jan 1, 1995Paper

L-lactate oxidase and L-lactate monooxygenase: mechanistic variations on a common structural theme

Biochimie
K Maeda-YoritaV Massey

Abstract

Properties of L-lactate oxidase from Aerococcus viridans are described. The gene encoding the enzyme has been isolated. From its cDNA sequence the amino acid sequence has been derived and shown to have high similarity with those of other enzymes catalyzing oxidation of L-alpha-hydroxy acids, including flavocytochrome b2, lactate monooxygenase, glycolate oxidase, mandelate dehydrogenases and a long chain alpha-hydroxy acid oxidase. The enzyme is expressed in Escherichia coli, and is a flavoprotein containing FMN as prosthetic group. It shares many properties of other alpha-hydroxy acid oxidizing enzymes, eg stabilization of the anionic semiquinone form of the flavin, facile formation of flavin-N(5)-sulfite adducts and a set of conserved amino acid residues around the bound flavin. Steady-state and rapid reaction kinetics of the enzyme have been studied and found to share many characteristics with those of L-lactate monooxygenase, but to differ from the latter in quantitative aspects. It is these quantitative differences between the two enzymes which account for the differences in the overall reactions catalyzed. These differences arise from different stabilities of a common intermediate of reduced flavin enzyme and pyruvate. In ...Continue Reading

References

Apr 1, 1992·Protein Science : a Publication of the Protein Society·F Lederer
Jul 1, 1992·The Biochemical Journal·C S MilesS K Chapman
Apr 20, 1990·Journal of Molecular Biology·Z X Xia, F S Mathews
Sep 1, 1989·Analytical Biochemistry·P BrissetteV Massey
Sep 5, 1989·Journal of Molecular Biology·Y Lindqvist
Oct 31, 1989·Biochemical and Biophysical Research Communications·J D DuncanM R Azari
Dec 15, 1988·European Journal of Biochemistry·G A ReidS K Chapman
Oct 1, 1986·The Biochemical Journal·S Ghisla, V Massey
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·Z X XiaF S Mathews
Oct 15, 1985·European Journal of Biochemistry·F LedererL Perez
Jan 1, 1994·Protein Science : a Publication of the Protein Society·A Balme, F Lederer
May 1, 1993·European Journal of Biochemistry·P MacherouxY Lindqvist
Aug 1, 1961·Archives of Biochemistry and Biophysics·M RIPPA

❮ Previous
Next ❯

Citations

Jul 31, 1998·Protein Science : a Publication of the Protein Society·K S Rao, F Lederer
Apr 20, 2011·European Biophysics Journal : EBJ·Florence Lederer
Sep 7, 2007·Applied Microbiology and Biotechnology·Cuiqing MaPing Xu
Mar 15, 2003·Biochimica Et Biophysica Acta·Giovanni Gadda
Sep 2, 1997·Proceedings of the National Academy of Sciences of the United States of America·K YoritaV Massey
Sep 18, 2009·The Journal of Biological Chemistry·Andrea Pennati, Giovanni Gadda
May 26, 2009·Acta Crystallographica. Section D, Biological Crystallography·Narayanasami SukumarF Scott Mathews
Mar 3, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Yasufumi UmenaYukio Morimoto
Dec 5, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Ingar LeirosEdward Hough
Jan 8, 2010·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Jean Marie BourhisYlva Lindqvist
Mar 10, 2009·Applied and Environmental Microbiology·Roslina A NawawiAndrew C Barnes
Jun 15, 2007·Journal of Bacteriology·Olivier BarréMarc Solioz
Feb 28, 2013·International Journal of Molecular Sciences·Karen Crozier-Reabe, Graham R Moran
Dec 24, 2013·Archives of Biochemistry and Biophysics·Mieke M E HuijbersWillem J H van Berkel
Mar 8, 2000·Proceedings of the National Academy of Sciences of the United States of America·K YoritaV Massey
Nov 15, 2000·Proceedings of the National Academy of Sciences of the United States of America·K YoritaV Massey
Aug 13, 2013·Natural Product Reports·Michael Richter
Sep 26, 2003·Journal of Enzyme Inhibition and Medicinal Chemistry·Sergio A StreitenbergerFrancisco García-Carmona
Nov 21, 2015·Journal of Molecular Biology·Nicholas FurnhamChristine A Orengo
Jan 17, 2009·Archives of Biochemistry and Biophysics·Kunchala Rungsrisuriyachai, Giovanni Gadda
Aug 3, 2007·Archives of Biochemistry and Biophysics·Caroline VignaudFlorence Lederer
May 23, 2007·Biochemical and Biophysical Research Communications·Shu Jie LiYukio Morimoto
Sep 30, 2006·Biochemical and Biophysical Research Communications·Yasufumi UmenaYukio Morimoto
Jul 15, 2015·Applied Microbiology and Biotechnology·Mathias PicklKurt Faber
Jun 22, 2014·Trends in Microbiology·Tianyi JiangPing Xu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.