PMID: 22574324May 11, 2012Paper

L-NAME co-treatment prevent oxidative damage in the lung of adult Wistar rats treated with vitamin A supplementation

Cell Biochemistry and Function
Matheus Augusto de Bittencourt PasqualiJosé Cláudio Fonseca Moreira

Abstract

Based on the fact that vitamin A in clinical doses is a potent pro-oxidant agent to the lungs, we investigated here the role of nitric oxide (NO•) in the disturbances affecting the lung redox environment in vitamin A-treated rats (retinol palmitate, doses of 1000-9000 IU•kg(-1)•day(-1)) for 28 days. Lung mitochondrial function and redox parameters, such as lipid peroxidation, protein carbonylation and the level of 3-nytrotyrosine, were quantified. We observed, for the first time, that vitamin A supplementation increases the levels of 3-nytrotyrosine in rat lung mitochondria. To determine whether nitric oxide (NO •) or its derivatives such as peroxynitrite (ONOO-) was involved in this damage, animals were co-treated with the nitric oxide synthase inhibitor L-NAME (30 mg•kg(-1), four times a week), and we analysed if this treatment prevented (or minimized) the biochemical disturbances resulting from vitamin A supplementation. We observed that L-NAME inhibited some effects caused by vitamin A supplementation. Nonetheless, L-NAME was not able to reverse completely the negative effects triggered by vitamin A supplementation, indicating that other factors rather than only NO• or ONOO- exert a prominent role in mediating the redox eff...Continue Reading

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Citations

Jan 13, 2016·European Journal of Nutrition·Rafael LonghiDiogo Onofre Souza
Dec 4, 2014·Molecular Neurobiology·Matheus Augusto de Bittencourt PasqualiJosé Cláudio Fonseca Moreira
Feb 2, 2013·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Ling TaoXiang Chun Shen
Jun 17, 2015·Oxidative Medicine and Cellular Longevity·Marcos Roberto de Oliveira

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