L-NAME-treatment alters ectonucleotidase activities in kidney membranes of rats

Life Sciences
Cristina Ribas FürstenauSusana Tchernin Wofchuk

Abstract

To investigate the effect of N(omega)-Nitro-L-arginine methyl ester (l-NAME) treatment, known to induce a sustained elevation of blood pressure, on ectonucleotidase activities in kidney membranes of rats. L-NAME (30 mg/kg/day) was administered to Wistar rats for 14 days in the drinking water. Enzyme activities were determined colorimetrically and their gene expression patterns were analyzed by semi-quantitative RT-PCR. The metabolism of ATP and the accumulation of adenosine were evaluated by HPLC in kidney membranes from control and hypertensive rats. PKC phosphorylation state was investigated by Western blot. We observed an increase in systolic blood pressure from 115+/-12 mmHg (control group) to 152+/-18 mmHg (l-NAME-treated group). Furthermore, the hydrolysis of ATP, ADP, AMP, and p-Nph-5'TMP was also increased (17%, 35%, 27%, 20%, respectively) as was the gene expression of NTPDase2, NTPDase3 and NPP3 in kidneys of hypertensive animals. Phospho-PKC was increased in hypertensive rats. The general increase in ATP hydrolysis and in ecto-5'-nucleotidase activity suggests a rise in renal adenosine levels and in renal autoregulatory responses in order to protect the kidney against the threat presented by hypertension.

References

Jul 15, 1992·The Biochemical Journal·H Zimmermann
Sep 1, 1986·Analytical Biochemistry·K M ChanK D Junger
Jun 3, 2000·American Journal of Physiology. Renal Physiology·R LemmensL Vanduffel
May 8, 2001·Clinical and Experimental Pharmacology & Physiology·E M Schwiebert
May 16, 2001·American Journal of Physiology. Renal Physiology·E W Inscho
Oct 19, 2001·The International Journal of Biochemistry & Cell Biology·V P VieiraM R Schetinger
Dec 26, 2001·Current Opinion in Nephrology and Hypertension·Pontus B Persson
Aug 2, 2002·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·John D Imig, Edward W Inscho
Nov 5, 2003·Journal of Hypertension·Judith A Whitworth, UNKNOWN World Health Organization, International Society of Hypertension Writing Group
Nov 14, 2003·News in Physiological Sciences : an International Journal of Physiology Produced Jointly by the International Union of Physiological Sciences and the American Physiological Society·Robert J UnwinGeoffrey Burnstock
Apr 2, 2004·Nephron. Physiology·M A BaileyR J Unwin
Apr 17, 2004·Kidney International·Helga VitzthumArmin Kurtz
Nov 19, 2004·International Review of Cytology·Geoffrey Burnstock, Gillian E Knight
Jan 6, 2005·American Journal of Physiology. Renal Physiology·Bellamkonda K KishoreSimon C Robson
Sep 1, 2005·The Journal of Laboratory and Clinical Medicine·Lilach O LermanClaudio Napoli
Sep 29, 2005·American Journal of Physiology. Renal Physiology·Renu M VekariaRobert J Unwin
Oct 13, 2005·Journal of Cardiovascular Pharmacology·Adriana Werner BalbinottBeatriz D'Agord Schaan
Mar 2, 2006·Pharmacological Reviews·Geoffrey Burnstock
May 18, 2006·American Journal of Physiology. Renal Physiology·Joseph SatrianoRoland C Blantz
Jun 23, 2006·Journal of the American Society of Nephrology : JASN·Renu M VekariaDavid G Shirley
Mar 17, 2007·Life Sciences·Fernanda Cenci VuadenCarla Denise Bonan
Jul 17, 2007·Cardiovascular & Hematological Agents in Medicinal Chemistry·Hidemasa KatsumiMitsuru Hashida
Aug 19, 2007·Neurochemistry International·Eduardo Pacheco RicoCarla Denise Bonan
Nov 6, 2007·American Journal of Physiology. Renal Physiology·Volker Vallon
Feb 19, 2008·European Journal of Pharmacology·Eduardo Luiz PedrazzaCarla Denise Bonan

❮ Previous
Next ❯

Citations

Nov 23, 2013·Purinergic Signalling·Geoffrey BurnstockMatthew A Bailey
Jul 10, 2012·Life Sciences·Guilherme CotomacciMaria Luiza M Barreto-Chaves
Nov 24, 2011·European Journal of Pharmacology·Fernanda C VuadenCarla D Bonan
Jun 9, 2016·Autonomic Neuroscience : Basic & Clinical·Geoffrey Burnstock, Andrzej Loesch
Dec 18, 2013·Pharmacological Reviews·Geoffrey Burnstock, Vera Ralevic
Aug 24, 2012·Molecular and Cellular Biochemistry·Andréia Machado CardosoMaria Rosa Chitolina Schetinger
Sep 8, 2012·Molecular and Cellular Biochemistry·Maja MiloševićAnica Horvat
Mar 23, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Mireille Flaure Metchi DonfackThéophile Dimo

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.