l-Peptide functionalized dual-responsive nanoparticles for controlled paclitaxel release and enhanced apoptosis in breast cancer cells

Drug Delivery
Shiwei NiuLimin Zhu

Abstract

Nanoparticles and macromolecular carriers have been widely used to increase the efficacy of chemotherapeutics, largely through passive accumulation provided by their enhanced permeability and retention effect. However, the therapeutic efficacy of nanoscale anticancer drug delivery systems is severely truncated by their low tumor-targetability and inefficient drug release at the target site. Here, the design and development of novel l-peptide functionalized dual-responsive nanoparticles (l-CS-g-PNIPAM-PTX) for active targeting and effective treatment of GRP78-overexpressing human breast cancer in vitro and in vivo are reported. l-CS-g-PNIPAM-PTX NPs have a relative high drug loading (13.5%) and excellent encapsulation efficiency (74.3%) and an average diameter of 275 nm. The release of PTX is slow at pH 7.4 and 25 °C but greatly accelerated at pH 5.0 and 37 °C. MTT assays and confocal experiments showed that the l-CS-g-PNIPAM-PTX NPs possessed high targetability and antitumor activity toward GRP78 overexpressing MDA-MB-231 human breast cancer cells. As expected, l-CS-g-PNIPAM-PTX NPs could effectively treat mice bearing MDA-MB-231 human breast tumor xenografts with little side effects, resulting in complete inhibition of tumor g...Continue Reading

References

Apr 1, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·Ho-Young HwangYong-Hee Kim
Sep 2, 2008·International Journal of Biological Macromolecules·Jing TangXiulin Zhu
May 19, 2009·International Journal of Pharmaceutics·Jie GaoYanqiang Zhong
Mar 2, 2011·International Journal of Pharmaceutics·Cunxian DuanQiang Zhang
May 26, 2011·The Journal of Physical Chemistry. B·B HuQ R Huang
Oct 30, 2014·ACS Applied Materials & Interfaces·Shreya GoelWeibo Cai
Jul 1, 2016·Science Translational Medicine·Yosi ShamayDaniel A Heller

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Citations

Nov 12, 2020·Materials·Rayssa de Sousa VictorRomualdo Rodrigues Menezes
Nov 2, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Masoud FarshbafPaul R Walker
May 7, 2021·ACS Applied Bio Materials·Babak MamnoonSanku Mallik
Jun 20, 2021·Carbohydrate Research·Tanaji Nandgude, Roshani Pagar

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Methods Mentioned

BETA
phage-display
acetylation
dynamic light scattering
transmission electron microscopy
fluorescence microscopy
flow cytometry
PCR
xenografts

Software Mentioned

Image J
SPSS

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