L-plastin regulates polarization and migration in chemokine-stimulated human T lymphocytes

The Journal of Immunology : Official Journal of the American Association of Immunologists
Michael FreeleyAideen Long

Abstract

Chemokines such as SDF-1α play a crucial role in orchestrating T lymphocyte polarity and migration via polymerization and reorganization of the F-actin cytoskeleton, but the role of actin-associated proteins in this process is not well characterized. In this study, we have investigated a role for L-plastin, a leukocyte-specific F-actin-bundling protein, in SDF-1α-stimulated human T lymphocyte polarization and migration. We found that L-plastin colocalized with F-actin at the leading edge of SDF-1α-stimulated T lymphocytes and was also phosphorylated at Ser(5), a site that when phosphorylated regulates the ability of L-plastin to bundle F-actin. L-plastin phosphorylation was sensitive to pharmacological inhibitors of protein kinase C (PKC), and several PKC isoforms colocalized with L-plastin at the leading edge of SDF-1α-stimulated lymphocytes. However, PKC ζ, an established regulator of cell polarity, was the only isoform that regulated L-plastin phosphorylation. Knockdown of L-plastin expression with small interfering RNAs demonstrated that this protein regulated the localization of F-actin at the leading edge of chemokine-stimulated cells and was also required for polarization, lamellipodia formation, and chemotaxis. Knockdow...Continue Reading

References

Jan 9, 1999·DNA and Cell Biology·C S LinT F Lue
Aug 14, 1999·The Journal of Biological Chemistry·J Wang, E J Brown
Oct 6, 2000·The New England Journal of Medicine·U H von Andrian, C R Mackay
Nov 6, 2002·Journal of Biochemistry·Naoaki Saito, Yasuhito Shirai
Nov 8, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mercedes ReyFrancisco Sánchez-Madrid
Jan 15, 2003·Journal of Leukocyte Biology·Yvonne SamstagGuido H Wabnitz
Jun 18, 2005·Acta Pharmacologica Sinica·Veerle DelanoteJan Gettemans
Sep 1, 2005·The Journal of Cell Biology·Roumen PankovKenneth M Yamada
Mar 10, 2006·Journal of Cell Science·Atsushi Suzuki, Shigeo Ohno
Oct 21, 2006·Nature Immunology·Matthew F Krummel, Ian Macara
Jan 30, 2007·Nature Reviews. Immunology·Daniel D BilladeauTimothy S Gomez
Mar 6, 2007·Advances in Enzyme Regulation·Yvonne Samstag, Martin Klemke
Mar 14, 2007·The Journal of Cell Biology·Audrey GérardJohn G Collard
Oct 20, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Eliana RealJérôme Delon
Dec 7, 2007·Immunology Letters·Laura Patrussi, Cosima T Baldari
Jan 12, 2008·Journal of Cell Science·Sarah Russell
Feb 1, 2008·Proceedings of the National Academy of Sciences of the United States of America·Vitold E GalkinEdward H Egelman
Feb 22, 2008·Journal of Cell Science·Verena KölschRichard A Firtel
Aug 20, 2008·Nature Immunology·Marcus Thelen, Jens V Stein
Aug 20, 2008·Nature Immunology·Shannon K BromleyAndrew D Luster
Aug 30, 2008·Cytokine·Tian JinDale Hereld
Dec 19, 2008·Biological Chemistry·Claudia StrossRalf Kubitz
Jan 23, 2009·The Biochemical Journal·Stephen G Ward, Federica M Marelli-Berg
Apr 1, 2007·Cell Adhesion & Migration·Concepción Gómez-Moutón, Santos Mañes
Jul 15, 2009·Experimental Biology and Medicine·Ronald W Matheny, Martin L Adamo
Aug 22, 2009·Nature Reviews. Immunology·Victor L J Tybulewicz, Robert B Henderson
Sep 25, 2009·Molecular Biology of the Cell·Jianxin A YuChristopher E Turner
Nov 26, 2009·American Journal of Physiology. Endocrinology and Metabolism·Alexandra C Newton
Mar 3, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sharon Celeste MorleyPaul M Allen
May 13, 2010·Journal of Biomolecular Screening·Michael FreeleyDara J Dunican

❮ Previous
Next ❯

Citations

Jul 25, 2014·BioMed Research International·Nuno RosaMarlene Barros
Dec 5, 2015·Frontiers in Immunology·Loïc DupréJavier Rey-Barroso
Nov 18, 2014·Biochemical Society Transactions·Aideen Long, Michael Freeley
Dec 17, 2016·Blood·Martin Guilliams, Bart N Lambrecht
Apr 13, 2017·The Journal of Cell Biology·Wei Yung DingRonen Zaidel-Bar
Oct 15, 2013·Immunological Reviews·Alexander Babich, Janis K Burkhardt
Mar 7, 2014·Infection and Immunity·Lauren E DeadySharon Celeste Morley
Mar 1, 2014·Journal of the American Society of Nephrology : JASN·Toshiro Fujita
Mar 22, 2019·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Yan WangHeng Zhao
Oct 18, 2013·Tissue Antigens·F LafouresseL Dupré
May 30, 2014·The Journal of Biological Chemistry·Seow Theng OngAideen Long
Jul 6, 2014·Pigment Cell & Melanoma Research·Swetlana MactierRichard I Christopherson
Oct 15, 2013·Immunological Reviews·Sharon Celeste Morley
Sep 25, 2014·European Journal of Immunology·Navin K Verma, Dermot Kelleher
Dec 4, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Maiti J LommelElisabeth Schaffner-Reckinger
Sep 11, 2019·Nature Communications·Emre BaltaYvonne Samstag
Jul 29, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Xiaolu XuYina H Huang
Jul 22, 2014·Journal of Periodontal Research·V Ö ÖztürkN Bostanci
Jun 24, 2017·Journal of Leukocyte Biology·Phillip J Stewart-HutchinsonSharon Celeste Morley
May 8, 2014·Journal of Leukocyte Biology·Esther Serrano-PertierraCarlos López-Larrea
Sep 29, 2020·Cancer Biomarkers : Section a of Disease Markers·Qingwen ZengZhengrong Li
Aug 17, 2018·Future Science OA·Jon P DayGeorge S Baillie
Nov 3, 2020·Frontiers in Immunology·Fiona K BakkeHelen Dooley
Feb 24, 2021·Cell Communication and Signaling : CCS·Raquel A C MachadoElisabeth Schaffner-Reckinger
Apr 11, 2021·Bone Research·Hanan AljohaniMeenakshi A Chellaiah
Apr 17, 2021·The Journal of Immunology : Official Journal of the American Association of Immunologists·Edgar P AnayaS Celeste Morley

❮ Previous
Next ❯

Related Concepts

Related Feeds

Acute viral rhinopharyngitis

Acute viral rhinopharyngitis, also known as "common cold", is an acute, self-limiting viral infection of the upper respiratory tract involving the nose, sinuses, pharynx and larynx. Discover the latest research on acute viral rhinopharyngitis here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Brain developing: Influences & Outcomes

This feed focuses on influences that affect the developing brain including genetics, fetal development, prenatal care, and gene-environment interactions. Here is the latest research in this field.

Related Papers

The Journal of Immunology : Official Journal of the American Association of Immunologists
Konrad PazdrakAlexander Kurosky
American Journal of Physiology. Cell Physiology
Rachel A ClearyDale D Tang
The Journal of Immunology : Official Journal of the American Association of Immunologists
Sharon Celeste MorleyPaul M Allen
Journal of Leukocyte Biology
Wulin AerbajinaiGriffin P Rodgers
© 2021 Meta ULC. All rights reserved