L-Proline catabolism by the high G + C Gram-positive bacterium Paenarthrobacter aurescens strain TC1

Antonie van Leeuwenhoek
C E Deutch

Abstract

Paenarthrobacter aurescens (formerly called Arthrobacter aurescens) strain TC1 is a high G + C Gram-positive aerobic bacterium that can degrade the herbicide atrazine. Analysis of its genome indicated strain TC1 has the potential to form a bifunctional PutA protein containing L-proline dehydrogenase and L-glutamate-γ-semialdehyde dehydrogenase (L-Δ1-pyrroline-5-carboxylate dehydrogenase) activities. P. aurescens strain TC1 grew well in minimal media with L-Proline as a supplemental nutrient, the nitrogen source, or the sole carbon and nitrogen source. Multicellular myceloids induced by NaCl or citrate also grew on L-proline. The specific activity of L-proline dehydrogenase in whole cells was higher whenever L-proline was added to the medium. Both L-proline dehydrogenase and L-glutamate-γ-semialdehyde dehydrogenase (L-Δ1-pyrroline-5-carboxylate dehydrogenase) activities were found primarily in a membrane fraction from exponential-phase cells. The two activities co-eluted from a Bio-Gel P-60 column after precipitation of proteins with ammonium sulfate and solubilization with 0.1% Tween 20. The PutA protein in the active fraction also oxidized 3,4-dehydro-DL-proline, but there was no activity with other L-proline analogues. When P...Continue Reading

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Methods Mentioned

BETA
light microscopy
protein assay

Software Mentioned

Network Protein Sequence Analysis
Swiss
Model
ExPASy
BLAST

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