PMID: 6397997Dec 1, 1984Paper

L-Pyroglutamyl-L-phenylalanyl-L-leucine-p-nitroanilide--a chromogenic substrate for thiol proteinase assay

Analytical Biochemistry
Filippova IYuV M Stepanov

Abstract

L-Pyroglutamyl-L-phenylalanyl-L-leucine-p-nitroanilide (PFLNA)--a convenient chromogenic substrate for assay of thiol proteinases papain, ficin, and bromelain--was prepared by enzymatic synthesis with chymotrypsin as a catalyst. The thiol proteinases hydrolyze PFLNA with the liberation of p-nitroaniline, estimated spectrophotometrically by its absorbance at 410 nm. The phenylalanine residue in the P2 position of PFLNA meets the specificity demands of thiol proteinases. The following values of Km were found for PFLNA hydrolysis: by papain, 0.34 mM; by ficin, 0.43 mM; by bromelain, 0.30 mM. This substrate was successfully applied to monitor thiol proteinase affinity chromatography on bacitracin-Sepharose, which resulted in a 2- to 4-fold purification from commercial preparations.

Citations

May 8, 1998·Bioscience, Biotechnology, and Biochemistry·T IchimuraS Maruyama
Jul 28, 2011·Prikladnaia biokhimiia i mikrobiologiia·T A RevinaT A Valueva
Sep 11, 2002·Toxicon : Official Journal of the International Society on Toxinology·Hiroshi Mashiko, Hidenobu Takahashi
Aug 5, 2008·Bioorganicheskaia khimiia·N V MozhinaS V Kostrov
Aug 5, 2008·Bioorganicheskaia khimiia·T A SemashkoI Iu Filippova
Sep 25, 2009·Parasitology Research·Karina M RebelloCarlos R Alves
Dec 31, 1992·Clinica Chimica Acta; International Journal of Clinical Chemistry·D HeinováI Rosival
Feb 7, 2012·Plant Physiology and Biochemistry : PPB·Tatiana A ValuevaSergeii V Benevolensky
Jul 22, 2010·Experimental Parasitology·Saulo C BourguignonCarlos R Alves
Sep 29, 2015·Biomacromolecules·Ramon WeishauptJulian Ihssen
Aug 24, 2006·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·K S VinokurovM A Belozersky
Jul 6, 2010·The International Journal of Biochemistry & Cell Biology·Juliano S ToledoAngela K Cruz
Jan 11, 2013·Biotechnology Research International·Rajendra PavanAjay Kumar
Apr 1, 2008·Proceedings of the National Academy of Sciences of the United States of America·Jorge A ZavalaMay R Berenbaum
Feb 21, 2013·Journal of Lipid Research·Luz CamachoTimothy M Thomson
Sep 4, 2018·Metallomics : Integrated Biometal Science·Mickaël V CherrierJuan C Fontecilla-Camps
Dec 15, 2006·Chembiochem : a European Journal of Chemical Biology·Pierre HaquetteGérard Jaouen
Dec 8, 1989·European Journal of Biochemistry·S MiyataH K Kihara
Jan 1, 1995·Journal of Protein Chemistry·T HarrachH R Maurer
Sep 25, 2012·Bioorganicheskaia khimiia·S A KuznetsovG N Rudenskaia
Jul 10, 2019·Biotechnology Letters·María J TorresSebastián A Trejo
Dec 4, 2019·Bioscience, Biotechnology, and Biochemistry·Jia LiuYoshiaki Kouzuma
Jun 23, 2011·Organic & Biomolecular Chemistry·Pierre HaquetteMichèle Salmain
Jun 15, 2018·Journal of Burn Care & Research : Official Publication of the American Burn Association·Alexandra SchulzJennifer Lynn Schiefer
Feb 11, 2009·Acta Biochimica Et Biophysica Sinica·Constanza LiggieriNora Priolo
Nov 17, 2020·Frontiers in Molecular Biosciences·Irina Y FilippovaElena N Elpidina
Dec 12, 2018·Analytical Biochemistry·Elena N ElpidinaIrina Y Filippova

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.