L-selectin is not essential for naive CD4 cell trafficking or development of primary responses in Peyer's patches

European Journal of Immunology
L M BradleyS R Watson

Abstract

We showed previously that L-selectin-dependent recirculation of naive CD4 cells is essential for development of primary responses in peripheral lymph nodes. Recent studies suggest that L-selectin is also required for lymphocyte entry into gut mucosal lymphoid tissues that include Peyer's patches and mesenteric lymph nodes. Here we show that anti-L-selectin antibody, MEL-14, inhibited homing of a rigorously purified, homogenous population of naive CD4 cells into both of these tissues as well as peripheral lymph nodes, directly demonstrating a role for this receptor in regulating entry into gut-associated sites. However, in intact animals, treatment with MEL-14 resulted in the loss of naive CD4 cells (CD45RBhi, CD44lo from peripheral lymph nodes but not Peyer's patches, whereas mesenteric lymph nodes were intermediate in this regard. In mice primed by parenteral immunization with keyhole limpet hemocyanin (KLH), primary CD4 responses were readily detected in both. Peyer's patches and mesenteric lymph nodes, and were not affected by exposure to MEL-14. Indeed, similar frequencies of KLH-specific CD4 cells were recovered from both of these tissues irrespective of MEL-14 treatment. The results indicate that interactions with L-selec...Continue Reading

References

Apr 1, 1992·European Journal of Immunology·C R MackayW R Hein
Dec 16, 1993·Nature·E L BergE C Butcher
Dec 1, 1994·The Journal of Experimental Medicine·L M BradleyS L Swain
Mar 1, 1995·The Journal of Cell Biology·R AlonT A Springer
Jan 1, 1995·Advances in Immunology·B A Imhof, D Dunon
Nov 1, 1993·Gastroenterology·M F Kagnoff
Jul 1, 1993·The Journal of Experimental Medicine·R F Bargatze, E C Butcher

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Citations

Oct 30, 1999·European Journal of Immunology·L M BradleyS R Watson
Jun 5, 2008·Inflammatory Bowel Diseases·Jesús Rivera-NievesKlaus Ley
May 23, 1998·Annual Review of Immunology·R W DuttonS L Swain
Nov 21, 1998·Cell Adhesion and Communication·S R Watson, L M Bradley
Jan 21, 2014·Biochimica Et Biophysica Acta·Brett BurkholderRuo-Pan Huang
Dec 7, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yotam E Bar-EphraimReina E Mebius
Dec 22, 1999·The Journal of Immunology : Official Journal of the American Association of Immunologists·F Lepault, M C Gagnerault

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