PMID: 2504276May 16, 1989Paper

L-serine analogues form Schiff base and quinonoidal intermediates with Escherichia coli tryptophan synthase

Biochemistry
K F HoubenM F Dunn

Abstract

Substrate analogues of L-serine have been found that react with the alpha 2 beta 2 complex of Escherichia coli tryptophan synthase. Upon reaction with alpha 2 beta 2, the analogues glycine, L-histidine, L-alanine, and D-histidine form chemical intermediates derived from reaction with enzyme-bound pyridoxal 5'-phosphate with characteristic UV-visible spectral bands. The spectra of the products of the glycine, L-histidine, and L-alanine reactions with alpha 2 beta 2 contain contributions from the external aldimine, the quinonoid species, and other intermediates along the catalytic pathway. Just as previously reported for the reaction of L-serine with beta 2 [Goldberg, M. E., York, S., & Stryer, L. (1968) Biochemistry 7, 3662-3667], the reactions of glycine, L-histidine, and L-alanine with the beta 2 form of tryptophan synthase yield spectra with no contributions from catalytic intermediates beyond the external aldimine. The kinetics of intermediate formation and comparisons of the time courses for the exchange of alpha-1H for solvent 2H catalyzed by alpha 2 beta 2 or beta 2 were found to be consistent with these assignments. Intermediates further along the tryptophan synthase catalytic pathway are stabilized to a greater degree i...Continue Reading

References

Nov 28, 1986·Science·D K Srivastava, S A Bernhard
Sep 1, 1971·Biochemical and Biophysical Research Communications·H Kumagai, E W Miles
Oct 1, 1967·Chemical & Pharmaceutical Bulletin·S Inoue
Mar 15, 1967·Journal of the American Chemical Society·Y Matsushima, A E Martell
Nov 1, 1981·European Journal of Biochemistry·A N Lane, K Kirschner
Aug 13, 1962·Biochimica Et Biophysica Acta·J A DEMOSS
Mar 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·I P CRAWFORD, J ITO

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Citations

Oct 24, 2009·Biochemistry·Adam T DierkersMichael F Dunn
Jan 1, 1990·Critical Reviews in Biochemistry and Molecular Biology·R Bentley
Nov 17, 2006·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Andrea Mozzarelli, Stefano Bettati
Dec 3, 2003·The Journal of Biological Chemistry·Cristy L GellingIan W Dawes

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