L-serine deaminase of Escherichia coli.

Journal of Bacteriology
L AlföldiE Kerekes

Abstract

The native l-serine deaminase (l-serine hydrolyase, deaminating, EC 4.2.1.13) of Escherichia coli K-12, which seems to be a very labile protein, is rather stable in concentrated solution. Dilution rapidly inactivates it, but in the presence of a saturating concentration of l-serine the molecule is protected from inactivation. It is a very specific enzyme; l-serine is the sole substrate with a K(m) value of 6.60 x 10(-3)m. d-Serine and l-cysteine are competitive inhibitors. Substrate saturation curves of the native enzyme show sigmoid shape, whereas the enzyme liberated from the bacteria in the presence of l-serine exhibits normal Michaelis-Menten kinetics.

References

Dec 1, 1955·Journal of Bacteriology·A B PARDEE, L S PRESTIDGE
Apr 1, 1960·Journal of Bacteriology·M BENZIMANI C GUNSALUS
May 1, 1965·Journal of Molecular Biology·J MONODJ P CHANGEUX
May 1, 1955·Journal of Bacteriology·W L BOYD, H C LICHSTEIN

❮ Previous
Next ❯

Citations

Aug 1, 1975·Genetical Research·T Y MándiI Béládi
Jan 26, 1970·FEBS Letters·L Alföldi, I Raskó
Dec 2, 2004·Acta Microbiologica Et Immunologica Hungarica·Gabriella SpenglerJ Molnár
Mar 1, 1991·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·J MolnárF Gutmann
Nov 1, 1992·Antonie van Leeuwenhoek·J MolnárM J Nakamura
Apr 1, 1978·Genetical Research·J MolnárI B Holland
Aug 16, 1971·European Journal of Biochemistry·I Raskó, L Alföldi
Sep 27, 2018·Biotechnology for Biofuels·Si-Yu LiYun-Peng Chao
Jun 1, 1985·Journal of Bacteriology·E B NewmanC Walker
Aug 1, 1993·Trends in Biochemical Sciences·R GrabowskiW Buckel
Nov 1, 2005·EcoSal Plus·Larry Reitzer
Apr 1, 1974·Journal of Bacteriology·S Isenberg, E B Newman
Dec 26, 1969·Science·J Adler

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biosynthetic Transformations

Biosyntheic transformtions are multi-step, enzyme-catalyzed processes where substrates are converted into more complex products in living organisms. Simple compounds are modified, converted into other compounds, or joined together to form macromolecules. Discover the latest research on biosynthetic transformations here.