PMID: 11340646May 8, 2001Paper

L-Serine regulates the activities of microglial cells that express very low level of 3-phosphoglycerate dehydrogenase, an enzyme for L-Serine biosynthesis

Journal of Neuroscience Research
H SugishitaJ Tanaka

Abstract

Microglia are well known to become activated during various kinds of neuropathological events. The factors that are responsible for the activation, however, are not fully determined. In the present study, L-Ser was shown to enhance production of nitric oxide (NO), interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF alpha) by lipopolysaccharide (LPS)-stimulated cultured rat microglial cells. L-Ser, however, did not enhance the expression of mRNAs encoding inducible NO synthase, IL-6 and TNF alpha. On the other hand, astrocytes did not depend on L-Ser for release of IL-6 and TNF alpha. The expression of an enzyme 3-phosphoglycerate dehydrogenase (3PGDH), which is essential for L-Ser biosynthesis from a glycolytic intermediate 3-phosphoglycerate, was investigated. As revealed by Western blotting and immunocytochemical staining, 3PGDH-protein expression in vitro was the highest in astrocytes, intermediate in neurons and the lowest in microglial cells. Semiquantitative RT-PCR showed that microglial cells expressed 3PGDH-mRNA at a lower level than astrocytes. In frozen sections from rat forebrain, only astrocytes were immunoreactive for 3PGDH. The present study suggested that L-Ser is able to modulate microglial function mainly...Continue Reading

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Citations

Jun 20, 2003·Journal of Neuroscience Research·Yasuhide KuwabaraJunya Tanaka
Jul 9, 2003·Archives of Histology and Cytology·Shigeki Furuya, Masahiko Watanabe
Apr 30, 2008·Movement Disorders : Official Journal of the Movement Disorder Society·Hiroyuki SomaHidenao Sasaki
Feb 26, 2011·Pflügers Archiv : European journal of physiology·Jimmy Van den EyndenJean-Michel Rigo
Sep 16, 2005·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Bo ZhangMasahiro Sakanaka
Aug 14, 2003·Brain Research·Takeshi TakaradaYukio Yoneda
Jan 23, 2004·Experimental Neurology·Kohji MoriJunya Tanaka
Feb 17, 2006·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·María Gabriela ChelujaGabriela Beatriz Acosta
Aug 8, 2021·International Journal of Molecular Sciences·Mohammed E ChoudhuryJunya Tanaka
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