L-tryptophan radical cation electron spin resonance studies: connecting solution-derived hyperfine coupling constants with protein spectral interpretations.

Journal of the American Chemical Society
Henry D ConnorRonald P Mason

Abstract

Fast-flow electron spin resonance (ESR) spectroscopy has been used to detect a free radical formed from the reaction of l-tryptophan with Ce (4+) in an acidic aqueous environment. Computer simulations of the ESR spectra from l-tryptophan and several isotopically modified forms strongly support the conclusion that the l-tryptophan radical cation has been detected by ESR for the first time. The hyperfine coupling constants (HFCs) determined from the well-resolved isotropic ESR spectra support experimental and computational efforts to understand l-tryptophan's role in protein catalysis of oxidation-reduction processes. l-Tryptophan HFCs facilitated the simulation of fast-flow ESR spectra of free radicals from two related compounds, tryptamine and 3-methylindole. Analysis of these three compounds' beta-methylene hydrogen HFC data along with equivalent l-tyrosine data has led to a new computational method that can distinguish between these two amino acid free radicals in proteins without dependence on isotope labeling, electron-nuclear double resonance, or high-field ESR. This approach also produces geometric parameters (dihedral angles for the beta-methylene hydrogens) that should facilitate protein site assignment of observed l-tr...Continue Reading

References

Sep 15, 1984·Biochemical Pharmacology·P R WestR P Mason
Jun 1, 1994·Journal of Magnetic Resonance. Series B·D R Duling
Apr 8, 2000·Free Radical Biology & Medicine·M R GuntherR P Mason
Nov 20, 2003·Journal of the American Chemical Society·Jeremiah E MillerHarry B Gray
Dec 11, 2003·The Journal of Biological Chemistry·Xiangbo ZhaoRichard S Magliozzo
Jul 9, 2004·Biophysical Journal·Dimitri A Svistunenko, Chris E Cooper
Mar 17, 2007·Journal of the American Chemical Society·Robin SibertBridgette A Barry

❮ Previous
Next ❯

Citations

Mar 21, 2013·Journal of the American Society for Mass Spectrometry·Andrii PiatkivskyiVictor Ryzhov
Oct 20, 2011·Journal of the American Chemical Society·Stefan StollR David Britt
Oct 5, 2010·Chemical Communications : Chem Comm·Pinhong ChenChaozhong Li
Mar 15, 2013·Proceedings of the National Academy of Sciences of the United States of America·Erik T YuklAimin Liu
Nov 15, 2011·Bioelectrochemistry·Nadav Ben-Dov, Rafi Korenstein
Apr 9, 2009·Free Radical Biology & Medicine·Marilyn EhrenshaftRonald P Mason
Jun 2, 2017·Physical Chemistry Chemical Physics : PCCP·Leonardo Muñoz-RugelesJuan Raúl Alvarez-Idaboy
Mar 30, 2016·Physical Chemistry Chemical Physics : PCCP·Daniel R KattnigP J Hore
Jan 20, 2011·Magnetic Resonance in Chemistry : MRC·Andrej StaškoVlasta Brezová
Mar 8, 2011·Physical Chemistry Chemical Physics : PCCP·Olga B MorozovaAlexandra V Yurkovskaya
Feb 9, 2011·Physical Chemistry Chemical Physics : PCCP·Caterina BerniniAdalgisa Sinicropi
Oct 30, 2009·The Journal of Physical Chemistry. a·Artur F IzmaylovMichael J Frisch
Jul 31, 2010·Journal of the American Chemical Society·Stefan StollR David Britt
Aug 19, 2015·The Journal of Physical Chemistry Letters·Hannah S Shafaat, Judy E Kim
Jun 17, 2020·Inorganic Chemistry·Shorok A M AbdelhameedTatjana N Parac-Vogt

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.