PMID: 11925469Apr 2, 2002Paper

L-type amino acid transporters in two intestinal epithelial cell lines function as exchangers with neutral amino acids

The Journal of Nutrition
S FragaPatrício Soares-da-Silva

Abstract

The present study examined the functional characteristics of the inward [(14)C]-L-leucine transporter in two intestinal epithelial cell lines (human Caco-2 and rat IEC-6). The uptake of [(14)C]-L-leucine was largely promoted through an energy-dependent and sodium-insensitive transporter, although a minor component of [(14)C]-L-leucine uptake ( approximately 15%) required extracellular sodium. [(14)C] -L-leucine uptake was insensitive to N-(methylamino)-isobutyric acid, but competitively inhibited by 2-aminobicyclo(2,2,1)-heptane-2-carboxylic acid (BCH). Both L- and D-neutral amino acids, but not acidic and basic amino acids, markedly inhibited [(14)C]-L-leucine accumulation. The efflux of [(14)C]-L-leucine was markedly increased (P < 0.05) by L-leucine and BCH, but not by L-arginine. In IEC-6 cells, but not in Caco-2 cells, the uptake of [(14)C]-L-leucine at acidic pH (5.0 and 5.4) was greater (P < 0.05) than at pH 7.4. In conclusion, it is likely that system B(0) might be responsible for the sodium-dependent uptake of L-leucine in Caco-2 and IEC-6 cells, whereas sodium-independent uptake of L-leucine may include system LAT1, whose activation results in transstimulation of L-leucine outward transfer.

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Citations

Oct 7, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Patrício Soares-Da-SilvaMaria João Bonifácio
Feb 3, 2011·The Journal of Membrane Biology·Pedro GonçalvesFátima Martel
Oct 19, 2016·IUBMB Life·Lucia MastrototaroJörg R Aschenbach
Mar 19, 2008·Molecular and Cellular Biochemistry·João S AmaralPatrício Soares-da-Silva
Jan 16, 2009·American Journal of Physiology. Cell Physiology·Raquel Martín-VenegasRuth Ferrer
Jul 17, 2016·Archives of Toxicology·Christy C Bridges, Rudolfs K Zalups
Jul 24, 2004·American Journal of Physiology. Renal Physiology·Patrício Soares-da-Silva, Maria Paula Serrão
May 13, 2010·Bioscience, Biotechnology, and Biochemistry·Kazuo ShimizuMakoto Shimizu
Nov 5, 2019·Frontiers in Neurology·Sebastiaan P van Kessel, Sahar El Aidy
Jul 29, 2008·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Eva M del AmoMarjo Yliperttula

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