PMID: 9448946Feb 4, 1998Paper

L-type and Ca2+ release channel-dependent hierarchical Ca2+ signalling in rat portal vein myocytes

Cell Calcium
S ArnaudeauJean Mironneau

Abstract

Ca2+ signalling events and whole-cell Ca2+ currents were analyzed in single myocytes from rat portal vein by using a laser scanning confocal microscope combined with the patch-clamp technique. In myocytes in which the intracellular Ca2+ store was depleted or Ca2+ release channels were blocked by 10 microM ryanodine, inward Ca2+ currents induced slow and sustained elevations of [Ca2+]i. These Ca2+ responses were suppressed by 1 microM oxodipine and by depolarizations to +120 mV, a potential close to the reversal potential for Ca2+ ions, suggesting that they reflected Ca2+ influx through L-type Ca2+ channels. With functioning intracellular Ca2+ stores, flash photolysis of caged Ca2+ gave rise to a small increase in [Ca2+]i with superimposed Ca2+ sparks, reflecting the opening of clustered Ca2+ release channels. Brief Ca2+ currents in the voltage range from -30 to +10 mV triggered Ca2+ sparks or macrosparks that did not propagate in the entire line-scan image. Increasing the duration of Ca2+ current for 100 ms or more allowed the trigger of propagating Ca2+ waves which originated from the same initiation sites as the caffeine-activated response. Both Ca2+ sparks and initiation sites of Ca2+ waves activated by Ca2+ currents were ob...Continue Reading

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Citations

Apr 1, 2000·British Journal of Pharmacology·P ViardJ Mironneau
Oct 16, 2001·The Journal of Physiology·J MironneauC Mironneau
Dec 12, 2012·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Fabrice DabertrandJoseph E Brayden
Oct 20, 2009·Cell Calcium·Richard D RainbowJohn G McCarron
May 12, 1998·The Journal of Physiology·M J Taggart, S Wray
Sep 10, 2004·American Journal of Physiology. Cell Physiology·Sohag N Saleh, Iain A Greenwood
May 5, 2005·Canadian Journal of Physiology and Pharmacology·Kevin S Thorneloe, Mark T Nelson
Nov 5, 2013·American Journal of Physiology. Heart and Circulatory Physiology·John J RehoSteven A Fisher
Mar 29, 2000·The Journal of Biological Chemistry·F CoussinJ Mironneau
Sep 20, 2000·Journal of Hypertension·M FürstenauM Gollasch
Feb 15, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Richard P Burt
Feb 27, 2004·The Journal of Biological Chemistry·Jean-Luc MorelJean Mironneau
Sep 27, 2015·American Journal of Physiology. Heart and Circulatory Physiology·Olan Jackson-WeaverNancy L Kanagy
Aug 18, 2001·Journal of Applied Physiology·K M Sanders
Aug 14, 1999·Japanese Journal of Pharmacology·Y ImaizumiM Watanabe
Jul 17, 1999·The American Journal of Physiology·F X BoittinJ Mironneau

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