PMID: 9438829Mar 28, 1998Paper

L-type Ca2+ current in fish cardiac myocytes: effects of thermal acclimation and beta-adrenergic stimulation

The Journal of Experimental Biology
Matti Vornanen

Abstract

A patch-clamp analysis of L-type Ca2+ current in ventricular myocytes of cold- and warm-acclimated rainbow trout (Oncorhynchus mykiss) and crucian carp (Carassius carassius) hearts was performed. Trout were acclimated at 4 and 17 degrees C and carp at 4 and 24 degrees C for a minimum of 4 weeks. Ventricular myocytes were isolated by enzymatic dissociation using collagenase and trypsin. Marked species-specific differences were noted in Ca2+ current density and its ss-adrenergic regulation. The density of basal Ca2+ current in crucian carp (6.9-7.4 pA pF-1) was almost double that of trout (4.2-4.5 pA pF-1) ventricular myocytes. Maximal beta-adrenergic stimulation increased Ca2+ current by approximately 2.3-fold in trout but by only 1.4-fold in crucian carp, so that Ca2+ current densities in the presence of 10 micromol l-1 isoprenaline were almost equal in trout (8.6-10.5 pA pF-1) and carp (9.6-10.4 pA pF-1) cardiac cells. Direct activation of adenylate cyclase by forskolin (10 micromol l-1) was also associated with similar interspecies differences in the stimulation of Ca2+ current. Thermal acclimation did not change either the density or the kinetics of L-type Ca2+ current in crucian carp ventricular myocytes. In trout cardiac c...Continue Reading

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