L-type calcium channel blockers reverse docetaxel and vincristine-induced multidrug resistance independent of ABCB1 expression in human lung cancer cell lines

Toxicology Letters
Ling-Yen ChiuGwo-Tarng Sheu

Abstract

Multidrug resistance (MDR) of cancer cells to cytotoxic drugs significantly impedes chemotherapeutic treatment. The purpose of this study is to characterize docetaxel (DOC) or vincristine (VCR) selected A549 and H1299 non-small cell lung cancer (NSCLC) sublines that exhibit MDR phenotypes and followed by re-sensitization study. Although all drug resistant sublines showed cross-resistance to DOC, VCR, and doxorubicin (DXR), the expression of ATP-binding cassette (ABC) transporter B1 (ABCB1) gene was found to be strongly induced in DOC but not in VCR resistant A549 sublines by quantitative reverse transcription real-time polymerase chain reaction (qRT-PCR). In DOC and VCR resistant H1299 sublines, moderate expression of ABCB1 was detected. The levels of ABCB1 protein and efflux activities were further examined by immunoblotting and rhodamin-123 staining assay. The results showed that both ABC and non-ABC mediated MDR are existed. Furthermore, verapamil (VER), an inhibitor of ABCB1 and an L-type calcium channel blocker, is capable of reversing the resistance in all drug-resistant sublines independent of ABCB1 expression. Importantly, VER only sensitizes resistant sublines but has no effect on parental cancer cells. Other L-type ca...Continue Reading

References

Apr 1, 1992·Trends in Pharmacological Sciences·S B Horwitz
Aug 1, 1996·Journal of Chemotherapy·C V Timcheva, D K Todorov
Oct 6, 1997·Proceedings of the National Academy of Sciences of the United States of America·J V ThottasseryJ D Schuetz
Sep 2, 1999·International Journal of Cancer. Journal International Du Cancer·M V Blagosklonny, T Fojo
Jan 19, 2000·Trends in Biochemical Sciences·R W JohnstoneM J Smyth
Sep 7, 2000·Current Opinion in Oncology·B TanL Ratner
Mar 10, 2001·The Journal of Clinical Investigation·O BährM Weller
May 15, 2001·The Journal of Biological Chemistry·R A JohnsonK W Scotto
Nov 17, 2001·Current Opinion in Pharmacology·K W WoodJ R Jackson
May 11, 2002·Breast Cancer Research and Treatment·Jack S K ChenKapil Mehta
Nov 7, 2003·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·R D Baird, S B Kaye
Nov 26, 2003·International Immunopharmacology·F A Fitzpatrick, Richard Wheeler
Aug 5, 2005·International Journal of Cancer. Journal International Du Cancer·Jinghua Tsai ChangGwo-Tarng Sheu
Dec 20, 2005·Seminars in Oncology·Antonio Tito Fojo, Michael Menefee
Jun 30, 2006·Cancer Science·Shinzaburo Noguchi
Feb 6, 2007·Biochemical Pharmacology·David J Triggle
Jun 19, 2007·Biochimica Et Biophysica Acta·Jean-Pierre GilletJosé Remacle
Jun 23, 2007·Nature Reviews. Cancer·Gregory R MonteithSarah J Roberts-Thomson
Dec 11, 2007·Biochimica Et Biophysica Acta·Barbara T McGroganAmanda McCann
Oct 15, 2008·Molecular Cancer Therapeutics·Richard F RiedelPhillip G Febbo
May 29, 2009·CA: a Cancer Journal for Clinicians·Ahmedin JemalMichael J Thun

❮ Previous
Next ❯

Citations

Apr 8, 2014·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Ming-Ju HsiehHui-Ling Chiou
Sep 6, 2011·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Anne S TsaoDavid J Stewart
Mar 6, 2012·Phytotherapy Research : PTR·Shuli ManChangxiao Liu
May 21, 2013·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Julia FredeMustafa B A Djamgoz
Apr 14, 2015·BioMed Research International·Paul J DavisShaker A Mousa
Apr 4, 2015·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Yuan-Zheng XiaLing-Yi Kong
Oct 25, 2013·Toxicological Sciences : an Official Journal of the Society of Toxicology·Ming-Ju HsiehHui-Ling Chiou
Oct 30, 2010·Molecular BioSystems·Raul FecheteBernd Mayer
Oct 23, 2012·The Journal of Biological Chemistry·D James Surmeier, Paul T Schumacker
Apr 17, 2013·Movement Disorders : Official Journal of the Movement Disorder Society·David Sulzer, D James Surmeier
Apr 1, 2014·Wiley Interdisciplinary Reviews. Membrane Transport and Signaling·Jörg StriessnigPetronel Tuluc
Jun 18, 2014·Brain : a Journal of Neurology·Elena DragicevicBirgit Liss
Apr 8, 2015·Pharmacoepidemiology and Drug Safety·Yakun LangYu Fan
Nov 21, 2017·Journal of Parkinson's Disease·Honglei Chen
Feb 28, 2019·Endocrinology·Mavis A A TenkorangRebecca L Cunningham
Jul 14, 2012·Movement Disorders : Official Journal of the Movement Disorder Society·David Sulzer, D James Surmeier
Aug 2, 2018·International Journal of Oncology·Qijiao ChengJingyu Xu
Nov 29, 2017·Breast Cancer : the Journal of the Japanese Breast Cancer Society·Zong-Lin ZhangSu-Rong Wang
Feb 5, 2013·Cancer Chemotherapy and Pharmacology·Ching-Yuan ChengGwo-Tarng Sheu
Feb 18, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Robert MallonIrwin Hollander
Jul 22, 2021·The FEBS Journal·Ling WuLiang Zhao

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Cancer Genomics (Keystone)

Cancer genomics approaches employ high-throughput technologies to identify the complete catalog of somatic alterations that characterize the genome, transcriptome and epigenome of cohorts of tumor samples. Discover the latest research using such technologies in this feed.

Breast Cancer: Chemo-Resistance

Some cancers are difficult to treat and aggressive including the "triple-negative" breast cancer. This type of cancer is chemoresistant even before chemotherapy begins. Here are the latest discoveries chemo-resistance in breast cancer.

Related Papers

Anti-cancer Agents in Medicinal Chemistry
Simona RomanoMaria Fiammetta Romano
European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)
Y LiangM Clynes
© 2022 Meta ULC. All rights reserved