(L)-Valine production with minimization of by-products' synthesis in Corynebacterium glutamicum and Brevibacterium flavum

Amino Acids
Xiaohu HouWeiguo Zhang

Abstract

Corynebacterium glutamicum ATCC13032 and Brevibacterium flavum JV16 were engineered for L-valine production by over-expressing ilvEBN ( r ) C genes at 31 °C in 72 h fermentation. Different strategies were carried out to reduce the by-products' accumulation in L-valine fermentation and also to increase the availability of precursor for L-valine biosynthesis. The native promoter of ilvA of C. glutamicum was replaced with a weak promoter MPilvA (P-ilvAM1CG) to reduce the biosynthetic rate of L-isoleucine. Effect of different relative dissolved oxygen on L-valine production and by-products' formation was recorded, indicating that 15 % saturation may be the most appropriate relative dissolved oxygen for L-valine fermentation with almost no L-lactic acid and L-glutamate formed. To minimize L-alanine accumulation, alaT and/or avtA was inactivated in C. glutamicum and B. flavum, respectively. Compared to high concentration of L-alanine accumulated by alaT inactivated strains harboring ilvEBN ( r ) C genes, L-alanine concentration was reduced to 0.18 g/L by C. glutamicum ATCC13032MPilvA△avtA pDXW-8-ilvEBN ( r ) C, and 0.22 g/L by B. flavum JV16avtA::Cm pDXW-8-ilvEBN ( r ) C. Meanwhile, L-valine production and conversion efficiency were ...Continue Reading

References

May 8, 1998·The Proceedings of the Nutrition Society·P M Jakeman
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Apr 27, 2002·Applied and Environmental Microbiology·Eva RadmacherLothar Eggeling
Sep 2, 2003·Journal of Biotechnology·Thomas Hermann
Jan 11, 2005·Applied and Environmental Microbiology·Veronika ElisákováStéphane Delaunay
Oct 1, 2005·Applied Microbiology and Biotechnology·Wolfgang LeuchtenbergerKarlheinz Drauz
Nov 5, 2005·Applied and Environmental Microbiology·Petra Peters-WendischLothar Eggeling
Jan 13, 2006·Trends in Biotechnology·Karin HammerPeter Ruhdal Jensen
Apr 1, 2006·Applied Microbiology and Biotechnology·Hartmut F ZimmermannMichael Binder
Feb 13, 2007·Applied and Environmental Microbiology·Bastian BlombachBernhard J Eikmanns
Apr 28, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jin Hwan ParkSang Yup Lee
Jul 10, 2007·Journal of Bacteriology·Christian SolemPeter R Jensen
Dec 7, 2007·Molecular Systems Biology·Kwang Ho LeeSang Yup Lee
Feb 12, 2008·Current Protocols in Molecular Biology·Lynn ThomasonAmos Oppenheim
Apr 2, 2008·Applied Microbiology and Biotechnology·Bastian BlombachBernhard J Eikmanns
Oct 22, 2008·Applied and Environmental Microbiology·Jan Marienhagen, Lothar Eggeling
Oct 22, 2009·Applied Microbiology and Biotechnology·Jin Hwan Park, Sang Yup Lee
Nov 17, 2009·Journal of Microbiological Methods·Daqing XuXiaoyuan Wang
Jun 28, 2011·Journal of Industrial Microbiology & Biotechnology·Xiaohu HouWeiguo Zhang

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Citations

Dec 18, 2012·Applied and Environmental Microbiology·Satoshi HasegawaHideaki Yukawa
May 13, 2014·Applied Microbiology and Biotechnology·Marco OldigesBastian Blombach
Jan 12, 2013·Microbial Biotechnology·Miroslav PátekJan Nešvera
Mar 25, 2016·Biotechnology and Applied Biochemistry·Wenjian MaXiaoyuan Wang
Mar 15, 2015·Metabolic Engineering·Cheng ChenXiaoyuan Wang
Dec 2, 2014·Applied Microbiology and Biotechnology·Qinjian ZhuZhenghong Xu
Jul 6, 2016·Journal of Industrial Microbiology & Biotechnology·Jianzhong XuWeiguo Zhang
May 4, 2017·Biotechnology and Bioengineering·HaiFeng SuFuRong Tan
Mar 30, 2018·Applied Microbiology and Biotechnology·Xiaoyuan WangPeter J Quinn
Jun 1, 2020·Biotechnology and Applied Biochemistry·Yanchao ZhangXiaoyuan Wang
Jun 15, 2020·Journal of Industrial Microbiology & Biotechnology·Ying-Yu WangWei-Guo Zhang
Aug 24, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Li-Yan FengWei-Guo Zhang
Nov 18, 2018·Journal of Industrial Microbiology & Biotechnology·Milin ZhanYe Ni
Mar 27, 2019·Microbial Cell Factories·Jiao ZhangYi-Xin Huo
Apr 27, 2019·International Journal of Molecular Sciences·Ying-Yu WangLi-Ming Liu
Sep 1, 2021·Microbial Cell Factories·Hui GaoZhiming Rao

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