L1014F-kdr Mutation in Indian Anopheles subpictus (Diptera: Culicidae) Arising From Two Alternative Transversions in the Voltage-Gated Sodium Channel and a Single PIRA-PCR for Their Detection

Journal of Medical Entomology
Om P SinghManoj K Das

Abstract

Leucine-to-phenylalanine substitution at residue L1014 in the voltage-gated sodium channel, target site of action for dichlorodiphenyltrichloroethane (DDT) and pyrethroids, is the most common knockdown resistance (kdr) mutation reported in several insects conferring resistance against DDT and pyrethroids. Here, we report presence of two coexisting alternative transversions, A>T and A>C, on the third codon position of L1014 residue in malaria vector Anopheles subpictus Grassi (species A) from Jamshedpur (India), both leading to the same amino acid substitution of Leu-to-Phe with allelic frequencies of 19 and 67%, respectively. A single primer-introduced restriction analysis-polymerase chain reaction (PIRA-PCR) was devised for the identification of L1014F-kdr mutation in An. subpictus resulting from either type of point mutation. Genotyping of samples with PIRA-PCR revealed high frequency (82%) of L1014F-kdr mutation in the study area.

References

Jan 1, 1994·Medical and Veterinary Entomology·S G SugunaV Dhanda
Mar 4, 2008·Tropical Medicine & International Health : TM & IH·Charles S WondjiS H P Parakrama Karunaratne
Feb 16, 2010·Acta Tropica·Goutam ChandraSoumendranath Chatterjee
Jan 4, 2013·PloS One·Frank D RinkevichJeffrey G Scott

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