PMID: 12782278Jun 5, 2003Paper

L63, the Drosophila PFTAIRE, interacts with two novel proteins unrelated to cyclins

Mechanisms of Development
A RascleD S Hogness

Abstract

L63 encodes a CDK-like protein homologous to the mammalian PFTAIRE. We showed previously that L63 provides a CDK-related function critical to development (Dev. Biol. 221 (2000) 23). We present here the first biochemical characterization of L63 kinase. In addition, we describe two novel Drosophila proteins, PIF-1 and PIF-2 (for PFTAIRE Interacting Factor-1 and -2), identified in a two-hybrid screen for their ability to interact with the amino-terminal region of L63. The full-length PIF-1 cDNA shows an unusual dicistronic organization. PIF-1A and PIF-1B (the L63 interactor) predicted proteins are expressed in vivo, and show a distinct expression profile during development. Interaction between L63 and PIF-1B was confirmed in vitro and in vivo. The role of this interaction remains to be demonstrated, but our data suggest that PIF-1B might serve as a regulator of L63.

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Citations

Jan 27, 2010·Genetics·Dongmei Liu, Russell L Finley
May 23, 2007·Proceedings of the National Academy of Sciences of the United States of America·Fang ShuJun Wu
Mar 14, 2007·The Journal of Cell Biology·Lai-Wa TamPaul A Lefebvre
Aug 12, 2010·Fly·Dongmei LiuRussell L Finley

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