LA-ICP-MS imaging in multicellular tumor spheroids - a novel tool in the preclinical development of metal-based anticancer drugs

Metallomics : Integrated Biometal Science
Sarah TheinerBernhard K Keppler

Abstract

A novel application of advanced elemental imaging offers cutting edge in vitro assays with more predictive power on the efficacy of anticancer drugs in preclinical development compared to two dimensional cell culture models. We propose LA-ICP-MS analysis of multicellular spheroids, which are increasingly being used as three dimensional (3D) models of tumors, for improving the in vitro evaluation of anticancer metallodrugs. The presented strategy is very well suited for screening drug-tumor penetration, a key issue for drug efficacy. A major advantage of tumor spheroid models is that they enable us to create a tissue-like structure and function. With respect to 2D culture on the one hand and in vivo models on the other, multicellular spheroids thus show intermediate complexity, still allowing high repeatability and adequate through-put for drug research. This strongly argues for the use of spheroids as bridging models in preclinical anticancer drug development. Probing the lateral platinum distribution within these tumor models allows visualizing the penetration depth and targeting of platinum-based complexes. In the present study, we show for the first time that spatially-resolved metal accumulation in tumor spheroids upon trea...Continue Reading

References

Aug 29, 2009·Chemical Reviews·Alice V Klein, Trevor W Hambley
Jan 26, 2010·Journal of Biotechnology·Franziska HirschhaeuserLeoni A Kunz-Schughart
May 23, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Geeta MehtaShuichi Takayama
Jul 17, 2012·Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS)·Valentina TrunovaBoris Churin
Aug 13, 2013·Metallomics : Integrated Biometal Science·Olga ReifschneiderUwe Karst
Jul 6, 2014·Metallomics : Integrated Biometal Science·Juliusz BiangaChristian Larroque
Nov 22, 2014·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Sarah TheinerBernhard K Keppler
Jun 21, 2015·Investigational New Drugs·Ekaterina Schreiber-BrynzakBernhard K Keppler

❮ Previous
Next ❯

Citations

Dec 6, 2016·Scientific Reports·Xin Liu, Amanda B Hummon
Feb 6, 2017·Metallomics : Integrated Biometal Science·Ronald F S LeePaul J Dyson
Jun 22, 2019·Rapid Communications in Mass Spectrometry : RCM·Naoyuki Nakada, Yasuo Kuroki
Nov 7, 2019·The Plant Journal : for Cell and Molecular Biology·Megan C SheldenStephen D Tyerman
Jun 12, 2020·Metallomics : Integrated Biometal Science·Sarah TheinerGunda Koellensperger
Nov 3, 2020·Dalton Transactions : an International Journal of Inorganic Chemistry·Sophia HarringerBernhard K Keppler
Dec 10, 2020·Metallomics : Integrated Biometal Science·Debora WernitznigBernhard K Keppler
May 7, 2021·Analytical and Bioanalytical Chemistry·Andreas SchweikertGunda Koellensperger
Oct 24, 2019·Analytical Chemistry·Mika T WesterhausenPhilip A Doble
Nov 23, 2019·Analytical Chemistry·Fernando TobiasAmanda B Hummon

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.