Lab-On-A-Chip Device for Yeast Cell Characterization in Low-Conductivity Media Combining Cytometry and Bio-Impedance

Sensors
Julien ClaudelDjilali Kourtiche

Abstract

This paper proposes a simple approach to optimize the operating frequency band of a lab-on-a-chip based on bio-impedance cytometry for a single cell. It mainly concerns applications in low-conductivity media. Bio-impedance allows for the characterization of low cell concentration or single cells by providing an electrical signature. Thus, it may be necessary to perform impedance measurements up to several tens of megahertz in order to extract the internal cell signature. In the case of single cells, characterization is performed in a very small volume down to 1 pL. At the same time, measured impedances increase from tens of kilo-ohms for physiological liquids up to several mega-ohms for low conductivity media. This is, for example, the case for water analysis. At frequencies above hundreds of kilohertz, parasitic effects, such as coupling capacitances, can prevail over the impedance of the sample and completely short-circuit measurements. To optimize the sensor under these conditions, a complete model of a cytometry device was developed, including parasitic coupling capacitances of the sensor to take into account all the impedances. It appears that it is possible to increase the pass band by optimizing track geometries and plac...Continue Reading

References

Aug 26, 1976·The Journal of Membrane Biology·K AsamiN Koizumi
Jul 30, 2014·IEEE Transactions on Medical Imaging·Ryan J HalterKeith D Paulsen
Nov 11, 2017·Lab on a Chip·N KolluriM Cabodi

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Citations

Oct 4, 2020·Sensors·Thomas ChalklenSohini Kar-Narayan
Dec 20, 2020·Biosensors·Julien ClaudelMustapha Nadi
Jan 12, 2021·ACS Sensors·Beckett C Colson, Anna P M Michel

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Methods Mentioned

BETA
biosensor

Software Mentioned

LabVIEW
COMSOL

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