Label-free biosensing of Salmonella enterica serovars at single-cell level

Journal of Nanobiotechnology
Bin WangYongkuk Kwon

Abstract

The emerging nanotechnologies have greatly facilitated the development of label-free biosensors. The atomic force microscopy (AFM) has been used to study the molecular mechanism of the reactions for protein and aptamers. The surface plasmon resonance (SPR) have been used in fast detections of various pathogens such as bacteria. This study used both AFM and SPR to investigate the complex reactions between aptamers and outer membrane proteins (OMPs) on the surface of S. typhimurium. Two DNA aptamers were used for the label-free detections of S. typhimurium by AFM and SPR. The aptamers have specific binding affinities to the OMPs of S. typhimurium. At single-molecule level, the high resolution AFM topography and recognition images distinguished the OMPs on the bacteria surface, which is the first time the location of individual outer membrane protein have been determined on Salmonella surface. E. coli in the control experiments didn't generate recognition signals, which proved the specificity of these two aptamers to S. typhimurium. The off-rate values for the interactions of these two aptamers to the OMPs were estimated as 5.2 × 10(-3) and 7.4 × 10(-3) s(-1), respectively, by the AFM dynamic force microscopy (DFS). The force and ...Continue Reading

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Citations

Feb 2, 2018·Advanced Materials·Yuezhou ZhangHongbo Zhang
Jul 24, 2021·Biotechnology and Applied Biochemistry·Sebnem SeherlerIlke Anac Sakir

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Methods Mentioned

BETA
atomic force microscopy
AFM
biosensors
surface plasmon resonance
biosensor
chip

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