Label-free characterization of collagen fibers in cancerous esophagus tissues using ratiometric nonlinear optical microscopy.

Experimental Biology and Medicine
Wei-Chung ChenGuan-Yu Zhuo

Abstract

The issue of classifying esophageal cancer at various developmental stages is crucial for determining the optimized treatment protocol for the patients, as well as the prognosis. Precision improvement in staging esophageal cancer keeps seeking quantitative and analytical imaging methods that could augment histopathological techniques. In this work, we used nonlinear optical microscopy for ratiometric analysis on the intrinsic signal of two-photon excited fluorescence (TPEF) and second harmonic generation (SHG) from single collagen fibers only in submucosa of esophageal squamous cell carcinoma (ESCC). The blind tests of TPEF/SHG and forward (F)/backward (B) SHG were demonstrated to compare with the histology conclusion. The discussion of sensitivity and specificity was provided via statistical comparison between the four stages of esophageal cancer. To the best of our knowledge, this is the first study of using these two ratios in combination for staging ESCC.

References

Aug 15, 2002·Proceedings of the National Academy of Sciences of the United States of America·Aikaterini ZoumiBruce J Tromberg
Nov 10, 2004·Biophysical Journal·Rebecca M WilliamsWatt W Webb
Apr 19, 2007·Diseases of the Esophagus : Official Journal of the International Society for Diseases of the Esophagus·M A KaraB C Wilson
Apr 24, 2007·Biophysical Journal·François LégaréBjorn R Olsen
Mar 5, 2009·Journal of Biomedical Optics·Shi-Wei ChuChi-Hung Lin
Sep 17, 2007·Optics Express·Shi-Wei ChuBai-Ling Lin
Mar 9, 2010·Journal of Structural Biology·Zong-Yan ZhuoShi-Wei Chu
Nov 4, 2010·Surgical Endoscopy·Jun YanMingang Ying
Oct 15, 2009·World Journal of Gastrointestinal Endoscopy·Rajvinder SinghKrish Ragunath
May 3, 2011·Journal of Biomedical Optics·Shulian WuShufei Xu
Jan 19, 2012·Biomedical Optics Express·Gaël LatourMarie-Claire Schanne-Klein
Jan 20, 2012·Annals of Biomedical Engineering·Seth W PerryEdward B Brown
Oct 9, 2012·Optics Letters·Xiyi ChenPaul J Campagnola
Jan 11, 2014·Journal of Biomedical Optics·Jeremy S BredfeldtKevin W Eliceiri
Jan 31, 2014·Archives of Pathology & Laboratory Medicine·Jianxin ChenDhanpat Jain
Apr 22, 2015·World Journal of Gastroenterology : WJG·Lian-Huang LiJian-Xin Chen
Mar 26, 2016·European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP)·Bhawna Gupta, Narinder Kumar
Jun 15, 2016·Lasers in Medical Science·Lianhuang LiJianxin Chen
Apr 28, 2017·Annals of Cardiothoracic Surgery·Shilpa Jain, Sadhna Dhingra
Feb 8, 2018·Lasers in Medical Science·Kexin HeZhining Fan

❮ Previous
Next ❯

Citations

Apr 9, 2021·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Chao-Wei HungGuan-Yu Zhuo

❮ Previous
Next ❯

Methods Mentioned

BETA
imaging techniques
confocal microscopy
light scattering
surgical resection
biopsies
Nonlinear optical microscopy
optical microscopy

Software Mentioned

ImageJ
Matlab

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery
Lei XuXiankai Chen
Journal of Biomedical Optics
Paul J CampagnolaL M Loew
Diseases of the Esophagus : Official Journal of the International Society for Diseases of the Esophagus
J ChenX Guan
Bio-medical Materials and Engineering
Mariana BianchiVíctor H Casco
© 2022 Meta ULC. All rights reserved