Label-free colorimetric detection of gelatinases on nanoporous silicon photonic films

Analytical Chemistry
Lizeng GaoDi Gao

Abstract

We report the development of a sensor platform for detection of gelatinases based on porous silicon photonic films. The sensor is made by spin-coating gelatin, a substrate protein to gelatinases, onto the porous silicon, which forms a thin, uniform, and smooth gel layer where samples can be directly spotted. The digestion products of gelatin by the active gelatinase present in the sample are able to enter the pores and induce color changes that can be detected by the naked eye. Using this sensor, we have demonstrated the detection of matrix metalloproteinase-2 (MMP-2)-an important gelatinase closely associated with tumor aggressiveness and metastatic potential-with concentrations varying from 0.1 to 1000 ng/mL in samples with volumes as small as 1 microL. The detection limit of this method, in terms of the minimum quantity of active MMP-2 in the sample that can be detected, is 2 orders of magnitude lower than what has been reported for zymography.

References

Sep 18, 1997·Journal of the National Cancer Institute·A F Chambers, L M Matrisian
May 13, 1998·Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology·G González-AvilaR Pérez-Tamayo
Oct 24, 1998·International Journal of Gynecological Pathology : Official Journal of the International Society of Gynecological Pathologists·B DavidsonR Reich
Aug 11, 2000·Matrix Biology : Journal of the International Society for Matrix Biology·V M BaragiS K Rao
May 23, 2001·The Journal of Surgical Research·X YangH E Appert
Nov 14, 2002·Laboratory Investigation; a Journal of Technical Methods and Pathology·Boris I RatnikovAlex Y Strongin
Mar 6, 2003·Nature Materials·Frédérique CuninMichael J Sailor
Oct 2, 2003·International Journal of Cancer. Journal International Du Cancer·Matthew G TuttonA Muti Abulafi
Aug 11, 2004·Analytical Biochemistry·Ursula Pieper-FürstFrieder W Scheller
Jul 22, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Johanna NikkolaSeppo Pyrhönen
Mar 16, 2006·British Journal of Cancer·F J G M KubbenH W Verspaget
Mar 23, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Aparna A KamatAnil K Sood
Feb 23, 2007·Current Pharmaceutical Design·Barbara Fingleton

❮ Previous
Next ❯

Citations

Apr 11, 2013·Sensors·Claudia Pacholski
Jan 16, 2014·Bioanalysis·Franco MazzeiCristina Tortolini
May 9, 2015·Applied Microbiology and Biotechnology·Gregor TeglGeorg M Guebitz
Oct 13, 2012·Biosensors & Bioelectronics·Tim R DargavilleNicolas H Voelcker
Sep 22, 2017·Current Medicinal Chemistry·Antonio FranceskoTzanko Tzanov
Mar 27, 2009·Chemical Communications : Chem Comm·Kristopher A KilianJ Justin Gooding
Nov 22, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Zhen LeiZhenxin Wang
Oct 17, 2017·Scientific Reports·Rathi SaravananArtur Schmidtchen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.