Label-free colorimetric detection of specific sequences in genomic DNA amplified by the polymerase chain reaction

Journal of the American Chemical Society
Huixiang Li, Lewis J Rothberg

Abstract

We document the surprising result that single-stranded DNA adsorbs on negatively charged gold nanoparticles (Au-nps) with a rate that depends on sequence length and temperature. After ss-DNA adsorbs on Au-nps, we find that the particles are stabilized against salt-induced aggregation. These observations can be rationalized on the basis of electrostatics and form the basis for a colorimetric assay to identify specific sequences and single nucleotide polymorphisms on polymerase chain reaction (PCR)-amplified DNA. The assay is label-free, requires no covalent modification of the DNA or Au-np surfaces, and takes on the sensitivity of PCR. Most important, binding of target and probe takes place in solution where hybridization occurs in less than 1 min. As an example, we test PCR-amplified genomic DNA from clinical samples for single nucleotide polymorphisms (SNPs) associated with a fatal arrhythmia known as long QT syndrome.

References

Aug 5, 1993·Nature·S P FodorC L Adams
Sep 25, 1998·Science·M BruchezA P Alivisatos
Mar 10, 2001·Nature·P A FutrealM R Stratton
Apr 3, 2001·Nature Biotechnology·B DubertretA J Libchaber
Mar 22, 2002·Bioconjugate Chemistry·Shinji SuedaKazuko Matsumoto
Aug 9, 2002·Journal of the American Chemical Society·Dustin J MaxwellShuming Nie
Jan 23, 2003·Journal of the American Chemical Society·Brent S GaylordGuillermo C Bazan
Jan 24, 2003·Nature·Errol C Friedberg
Jan 24, 2003·Nature·Leroy Hood, David Galas
Jul 3, 2003·Journal of the American Chemical Society·Kae SatoMizuo Maeda
Oct 9, 2003·Journal of the American Chemical Society·Raj Chakrabarti, Alexander M Klibanov
Oct 24, 2003·Journal of Interventional Cardiac Electrophysiology : an International Journal of Arrhythmias and Pacing·Silvia G PrioriAlessandro Vicentini
Jun 14, 1999·Angewandte Chemie·Colin J LowethPeter G Schultz

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Citations

Nov 4, 2006·Analytical and Bioanalytical Chemistry·Z-H Mo, X-L Wei
Dec 25, 2007·Analytical and Bioanalytical Chemistry·Pedro BaptistaRicardo Franco
Jul 31, 2009·Analytical and Bioanalytical Chemistry·Zhang ChenQingyun Cai
Aug 26, 2011·Analytical and Bioanalytical Chemistry·Laura Maria ZanoliGiuseppe Spoto
Sep 10, 2013·Biosensors & Bioelectronics·Honghong YinChuanlai Xu
Nov 2, 2013·Biosensors & Bioelectronics·Tian TianEun-Cheol Lee
Nov 12, 2013·Biosensors & Bioelectronics·Sheng ChengMichael Hon-Wah Lam
Apr 27, 2011·Analytical Chemistry·Danny van LieropDuncan Graham
Jul 12, 2012·Analytical Chemistry·Yousef AkhlaghiMohammad Reza Hormozi-Nezhad
Jun 16, 2012·ACS Applied Materials & Interfaces·Jinzhao SongShu Wang
Jun 6, 2013·ACS Applied Materials & Interfaces·Xinhui Lou, Ying Zhang
Jun 12, 2013·ACS Applied Materials & Interfaces·Jing YangCheng Zhang
Jul 19, 2013·ACS Applied Materials & Interfaces·Rachel PautlerJuewen Liu
Oct 1, 2013·ACS Applied Materials & Interfaces·Cuifeng JiangQing-Hua Xu
Jul 13, 2012·Accounts of Chemical Research·Xiaoji XieXiaogang Liu
Mar 24, 2009·Chemical Reviews·Juewen LiuYi Lu
Feb 3, 2012·Chemical Reviews·Krishnendu SahaVincent M Rotello
Dec 13, 2012·Chemical Reviews·Hongquan ZhangX Chris Le
Aug 29, 2012·Journal of the American Chemical Society·Wei ShenZhiqiang Gao
Nov 4, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Yifan WangBin Liu
Sep 3, 2008·Chemical Society Reviews·Robert Wilson
May 9, 2008·Chemical Communications : Chem Comm·Chi-Wei LiuHuan-Tsung Chang
Oct 22, 2008·Chemical Communications : Chem Comm·Shijiang HeChunhai Fan
Mar 19, 2009·Chemical Communications : Chem Comm·Aiping XinLiansheng Ling
May 6, 2010·Chemical Communications : Chem Comm·Jeremy D Driskell, Ralph A Tripp

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