DOI: 10.1101/462002Nov 5, 2018Paper

Label-Free Density-Based Detection of Adipocytes of Bone Marrow Origin Using Magnetic Levitation

BioRxiv : the Preprint Server for Biology
O. SarigilEngin Ozcivici

Abstract

Adipocyte hypertrophy and hyperplasia are important parameters in describing abnormalities in adipogenesis that are concomitant to diseases such as obesity, diabetes, anorexia nervosa and osteoporosis. Therefore, technical developments in detection of adipocytes become an important driving factor in adipogenesis research. Current techniques such as optical microscopy and flow cytometry are available in detection and examination of adipocytes, driving cell- and molecular-based research of adipogenesis. Even though microscopy techniques are common and straightforward, they are restricted for manipulation and separation of the cells. Flow cytometry is an alternative, but mature adipocytes are fragile and cannot withstand the flow process. Other separation methods usually require labeling of the cells or usage of microfluidic platforms that utilize fluids with different densities. Magnetic levitation is a novel label-free technology with the principle of movement of cells towards the lower magnetic field in a paramagnetic medium depending on their individual densities. In this study, we used magnetic levitation device for density-based single cell detection of differentiated adipogenic cells in heterogeneous populations. Results sh...Continue Reading

Citations

Jan 9, 2019·Frontiers in Bioengineering and Biotechnology·Sena YamanH Cumhur Tekin

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BETA
optical microscopy

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ImageJ Fiji
OEK

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