Label-free detection of DNA hybridization at a liquid|liquid interface

Analytical Chemistry
Mikhail Yu VaginMarco Mascini

Abstract

A novel electrochemical approach for label-free detection of DNA primary sequence has been proposed. The flow of nonelectroactive ions across a liquid|liquid interface was used as an electrochemical probe for detection of DNA hybridization. Disposable graphite screen-printed electrodes shielded with a thin layer of inert polymer plasticized with water-immiscible polar organic solvent were modified by probe oligonucleotide and used as a DNA sensor. The specific DNA coupling has been detected with impedance spectroscopy by decrease of ion-transfer resistance. The detection limit was of 10-8 M of target oligonucleotide. The reported sensor was suitable for discrimination of a single mismatch oligonucleotide from the full complementary one. The reported DNA sensor was advantageous over known physicochemical approaches, providing the most significant changes in the measured parameters.

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Citations

Feb 3, 2012·Analytical and Bioanalytical Chemistry·Patoommarn WipawakarnDanny K Y Wong
Aug 10, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Napoleon TerceroRastislav Levicky
Jul 20, 2010·Annual Review of Analytical Chemistry·Byoung-Yong Chang, Su-Moon Park
Jun 13, 2018·Analytical Chemistry·Bren Mark B Felisilda, Damien W M Arrigan

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