Label-free detection of live cancer cells and DNA hybridization using 3D multilayered plasmonic biosensor

Nanotechnology
Shuyan ZhuStella W Pang

Abstract

Three-dimensional (3D) multilayered plasmonic nanostructures consisting of Au nanosquares on top of SU-8 nanopillars, Au asymmetrical nanostructures in the middle, and Au asymmetrical nanoholes at the bottom were fabricated through reversal nanoimprint technology. Compared with two-dimensional and quasi-3D plasmonic nanostructures, the 3D multilayered plasmonic nanostructures showed higher electromagnetic field intensity, longer plasmon decay length and larger plasmon sensing area, which are desirable for highly sensitive localized surface plasmonic resonance biosensors. The sensitivity and resonance peak wavelength of the 3D multilayered plasmonic nanostructures could be adjusted by varying the offset between the top and bottom SU-8 nanopillars from 31% to 56%, and the highest sensitivity of 382 and 442 nm/refractive index unit were observed for resonance peaks at 581 and 805 nm, respectively. Live lung cancer A549 cells with a low concentration of 5 × 103 cells ml-1 and a low sample volume of 2 μl could be detected by the 3D multilayered plasmonic nanostructures integrated in a microfluidic system. The 3D plasmonic biosensors also had the advantages of detecting DNA hybridization by capturing the complementary target DNA in t...Continue Reading

References

Dec 24, 2004·Lab on a Chip·Seog Woo RheeNoo Li Jeon
Nov 7, 2006·Proceedings of the National Academy of Sciences of the United States of America·Matthew E StewartJohn A Rogers
May 24, 2008·Nature Materials·Jeffrey N AnkerRichard P Van Duyne
Jan 23, 2009·Optics Express·Basudev LahiriNigel P Johnson
Jun 9, 2011·Chemical Reviews·Kathryn M Mayer, Jason H Hafner
Dec 26, 2013·Sensors·Ndubuisi E J OmaghaliGiancarlo Abbate
Mar 8, 2014·ACS Nano·Hyeun Joong YoonSunitha Nagrath
Apr 16, 2014·Nano Letters·Srdjan S AćimovićRomain Quidant
Oct 4, 2014·Science·Philip D HowesMolly M Stevens
Jan 27, 2015·The Journal of Physical Chemistry. C, Nanomaterials and Interfaces·Reid E MessersmithScott M Reed
Apr 18, 2015·Science·Mark I Stockman
Jul 3, 2015·ACS Nano·Eden Morales-NarváezArben Merkoçi
Aug 2, 2015·Biosensors & Bioelectronics·Xinghai ZhaoStella W Pang
Oct 21, 2016·Journal of Colloid and Interface Science·Antonino RapisardaGiovanni Marletta
Sep 2, 2017·Frontiers in Immunology·Yuanhao XuStella W Pang
Jan 31, 2018·ACS Sensors·Lisa P HackettGang Logan Liu
Mar 5, 2018·Talanta·Erin E BedfordFrank X Gu
Jul 21, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·M RippaL Petti

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Citations

May 28, 2019·Journal of Nanobiotechnology·Monika RuzyckaIreneusz P Grudzinski
May 19, 2020·Lab on a Chip·Shuyan ZhuStella W Pang
Mar 3, 2020·Talanta·Majid SharifiMojtaba Falahati
Sep 9, 2021·Mikrochimica Acta·J Rafaela L GuerreiroMarta Prado

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