Label-free detection of pathogenic bacteria via immobilized antimicrobial peptides

Talanta
Zong-Mu Dong, Guang-Chao Zhao

Abstract

A novel label-free strategy for the detection of bacteria was developed by using a specific antimicrobial peptide (AMP)-functionalized quartz crystal microbalance (QCM) electrode. This electrode interface was successfully applied to detect pathogenic Escherichia coli O157:H7 based on the specific affinity between the small synthetic antimicrobial peptide and the bacterial cell of pathogenic E. coli O157:H7. The concentrations of pathogenic E. coli O157:H7 were sensitively measured by the frequency response of the QCM with a detection limit of 0.4 cfu μL(-1). The detection can be fulfilled within 10 min because it does not require germiculture process. On the other hand, if the specific antimicrobial peptides were immobilized on a gold electrode, this label-free strategy can also be performed by electrochemical impedance spectroscopy (EIS). Compared with QCM technique, the EIS measurement gives a lower sensitivity and needs a longer assay time. The combination of antimicrobial peptides with the real-time responses of QCM, as well as electronic read-out monitoring of EIS, may open a new way for the direct detection of bacteria.

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Citations

Dec 15, 2015·Advanced Materials·Amanda L Bole, Panagiotis Manesiotis
May 18, 2016·Analytica Chimica Acta·Kazuharu SugawaraHideki Kuramitz
Jul 12, 2016·Biosensors & Bioelectronics·Mireia Hoyos-NoguésFrancisco-Javier Gil
Jul 13, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mireia Hoyos-NoguésCarlos Mas-Moruno
Aug 7, 2017·Technology·Fatemeh JalaliDaniel Irimia
Apr 30, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Éric PardouxYoann Roupioz
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mihaela TertisCecilia Cristea

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