Label-free detection of single nanoparticles and biological molecules using microtoroid optical resonators

Light, Science & Applications
Judith SuBrian M Stoltz

Abstract

Single-molecule detection is one of the fundamental challenges of modern biology. Such experiments often use labels that can be expensive, difficult to produce, and for small analytes, might perturb the molecular events being studied. Analyte size plays an important role in determining detectability. Here we use laser-frequency locking in the context of sensing to improve the signal-to-noise ratio of microtoroid optical resonators to the extent that single nanoparticles 2.5 nm in radius, and 15.5 kDa molecules are detected in aqueous solution, thereby bringing these detectors to the size limits needed for detecting the key macromolecules of the cell. Our results, covering several orders of magnitude of particle radius (100 nm to 2 nm), agree with the 'reactive' model prediction for the frequency shift of the resonator upon particle binding. This confirms that the main contribution of the frequency shift for the resonator upon particle binding is an increase in the effective path length due to part of the evanescent field coupling into the adsorbed particle. We anticipate that our results will enable many applications, including more sensitive medical diagnostics and fundamental studies of single receptor-ligand and protein-prot...Continue Reading

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Citations

Oct 13, 2018·Advanced Materials·Shui-Jing TangYun-Feng Xiao
Mar 14, 2019·Nanoscale·Zhang ZhangLimin Tong
May 24, 2016·Reports on Progress in Physics·Euan McLeod, Aydogan Ozcan
Feb 23, 2017·The Analyst·Angelo BozzolaFrancesco De Angelis
Jul 16, 2016·Annual Review of Biomedical Engineering·Aydogan Ozcan, Euan McLeod
Sep 14, 2017·Sensors·Weeratouch Pongruengkiat, Suejit Pechprasarn
Sep 16, 2020·Analytical Methods : Advancing Methods and Applications·Zhijun GuoHansong Yu
Jan 7, 2017·Advanced Materials·Yanyan ZhiYun-Feng Xiao
Jul 27, 2017·Lab on a Chip·Hyungsoon ImCesar M Castro
Mar 7, 2019·Light, Science & Applications·Xiao-Chong YuYun-Feng Xiao
Mar 7, 2019·Light, Science & Applications·Judith Su
Jan 10, 2020·Luminescence : the Journal of Biological and Chemical Luminescence·Xiao LiuZhi-Jun Ding
Jun 21, 2016·Sensors·Giancarlo C Righini, Silvia Soria
Aug 12, 2018·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Gudepalya Renukaiah Rudramurthy, Mallappa Kumara Swamy
Nov 15, 2018·Micromachines·Zhiqing Feng, Lan Bai
Nov 2, 2019·Optics Express·Z ZhangA Meldrum
Oct 23, 2020·Sensors·Donggee RhoSeunghyun Kim
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Feb 26, 2020·Biosensors & Bioelectronics·Su WangZhiyang Li
Aug 25, 2020·Matter·Xuefeng JiangLan Yang
Apr 1, 2021·Nature Communications·Ming JinYun-Feng Xiao
Jun 4, 2021·ACS Sensors·Sartanee SuebkaJudith Su
Jun 18, 2021·Light, Science & Applications·Da-Quan YangYun-Feng Xiao
May 28, 2019·ACS Sensors·Jiawei WangOliver G Schmidt

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Methods Mentioned

BETA
enzyme-linked immunosorbent assays
biosensing
scanning tunneling microscopy
chip
X-ray
biosensor

Software Mentioned

FLOWER
COMSOL

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