Label free electrochemical DNA hybridization discrimination effects at the binary and ternary mixed monolayers of single stranded DNA/diluent/s in presence of cationic intercalators

Biosensors & Bioelectronics
V Dharuman, Jong Hoon Hahn

Abstract

Electrochemical label free DNA hybridization discrimination of the brain tumor sequence CK20 has been made at the gold-thiol and thiol diluent binary and ternary mixed monolayer interfaces in presence of the [Fe(CN)6](3-) and double stranded DNA (dsDNA) specific cationic intercalators, proflavine (PF) and methylene blue (MB), respectively. Thiol hexane labeled single stranded DNA (HS-ssDNA) and thiol diluents such as 6-mercapto-1-hexanol (MCH) and 3-mercaptopropionic acid (MPA) are used to construct the mixed monolayers. Change in the peak-to-peak separation (Delta Ep) for the [Fe(CN)6](3-) redox reaction indicates the efficiency of the diluents in removing the randomly oriented HS-ssDNA. Smaller Delta Ep 248 mV noticed for the HS-ssDNA/MPA compared to the HS-ssDNA/MCH mixed monolayers (812 mV) indicates the less influence of the MCH diluent on the arrangement of HS-ssDNA layer. However, the hybridization discrimination effect negotiated in presence of both the [Fe(CN)6](3-) and PF intercalator showed zero effect for the HS-ssDNA/MPA interface, and approximately 20-30% effect for the HS-ssDNA/MCH interface. The discrimination effect at the HS-ssDNA/MPA interface further increased to 80% by inserting the MCH at the local defects...Continue Reading

References

Jan 1, 1997·Bioconjugate Chemistry·S O KelleyM G Hill
Nov 26, 1999·Nucleic Acids Research·S O KelleyM G Hill
Jul 29, 2000·Accounts of Chemical Research·A BadiaL Reven
Oct 4, 2000·Nature Biotechnology·E M BoonJ K Barton
Jan 29, 2002·Nucleic Acids Research·A W PetersonR M Georgiadis
Jun 26, 2002·Biochemistry·Elizabeth M BoonJacqueline K Barton
Mar 27, 2003·Journal of the American Chemical Society·Fredrik WesterlundPer Lincoln
Apr 24, 2003·Journal of the American Chemical Society·Dmitri Y PetrovykhMichael J Tarlov
Sep 25, 2003·Analytical and Bioanalytical Chemistry·Joerg AlbersRainer Hintsche
Oct 2, 2003·Nature Biotechnology·T Gregory DrummondJacqueline K Barton
Oct 16, 2003·Proceedings of the National Academy of Sciences of the United States of America·Elizabeth M BoonJacqueline K Barton
Feb 5, 2004·Journal of the American Chemical Society·Biao LiuStephen E Creager
Feb 14, 2004·Analytical Chemistry·Nokyoung Park, Jong Hoon Hahn
Sep 17, 2004·Journal of the American Chemical Society·Hiromi Kimura-SudaLloyd J Whitman
Apr 2, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Hainer WackerbarthJens Ulstrup
Apr 14, 2005·Chemical Reviews·J Christopher LoveGeorge M Whitesides
Jul 13, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Elicia L S WongJ Justin Gooding
Jul 21, 2005·Journal of the American Chemical Society·Tao Liu, Jacqueline K Barton
Oct 1, 2005·Analytical Chemistry·Ruojun LaoChunhai Fan
Apr 1, 2006·Biosensors & Bioelectronics·V DharumanR Hintsche
Apr 4, 2006·Analytical Chemistry·Elicia L S Wong, J Justin Gooding
Apr 19, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Lawrence Yoon Suk LeeR Bruce Lennox
May 3, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Christina BoozerShaoyi Jiang
Nov 30, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Rebecca Y LaiKevin W Plaxco

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Citations

Dec 25, 2009·Analytical and Bioanalytical Chemistry·Hany NasefCiara K O'Sullivan
Feb 3, 2012·Analytical and Bioanalytical Chemistry·Patoommarn WipawakarnDanny K Y Wong
Mar 1, 2012·Chemical Reviews·Emil Paleček, Martin Bartošík
Dec 4, 2014·Colloids and Surfaces. B, Biointerfaces·Yanli YangBo Liedberg
Apr 1, 2014·Colloids and Surfaces. B, Biointerfaces·Arzum Erdem, Gulsah Congur
Jan 24, 2019·International Journal of Molecular Sciences·Susana CampuzanoJosé M Pingarrón
Nov 23, 2011·Chemical Society Reviews·Zimple MatharuBansi Dhar Malhotra
May 31, 2017·Analytical and Bioanalytical Chemistry·Thu Huong HoMathieu Lazerges

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