Label-free electrochemical impedance biosensor to detect human interleukin-8 in serum with sub-pg/ml sensitivity.

Biosensors & Bioelectronics
R SharmaC Wälti

Abstract

Biosensors with high sensitivity and short time-to-result that are capable of detecting biomarkers in body fluids such as serum are an important prerequisite for early diagnostics in modern healthcare provision. Here, we report the development of an electrochemical impedance-based sensor for the detection in serum of human interleukin-8 (IL-8), a pro-angiogenic chemokine implicated in a wide range of inflammatory diseases. The sensor employs a small and robust synthetic non-antibody capture protein based on a cystatin scaffold that displays high affinity for human IL-8 with a KD of 35 ± 10 nM and excellent ligand specificity. The change in the phase of the electrochemical impedance from the serum baseline, ∆θ(ƒ), measured at 0.1 Hz, was used as the measure for quantifying IL-8 concentration in the fluid. Optimal sensor signal was observed after 15 min incubation, and the sensor exhibited a linear response versus logarithm of IL-8 concentration from 900 fg/ml to 900 ng/ml. A detection limit of around 90 fg/ml, which is significantly lower than the basal clinical levels of 5-10 pg/ml, was observed. Our results are significant for the development of point-of-care and early diagnostics where high sensitivity and short time-to-resul...Continue Reading

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Citations

Nov 11, 2016·Biosensors & Bioelectronics·Scarlet Xiaoyan WangRichard H Bayford
Feb 22, 2017·Chemical Communications : Chem Comm·Irene ArrataAndrew J Wilson
Oct 11, 2017·Sensors·Yue Cui
Sep 8, 2017·Sensors·José Juan-ColásThomas F Krauss
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Oct 22, 2019·Chembiochem : a European Journal of Chemical Biology·Sandeep KaushalDong Kee Yi
Aug 4, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Tong ShuMichael J Serpe
Sep 27, 2018·Analytical and Bioanalytical Chemistry·Raluca-Ioana Stefan-van StadenAdrian Streinu-Cercel
Sep 3, 2020·Nature Communications·Mukhil RaveendranPaolo Actis
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Jul 30, 2020·Analytical and Bioanalytical Chemistry·Dipti RaniVivek Pachauri
Jan 14, 2021·Scientific Reports·Shazana Hilda ShamsuddinPaul A Millner
Jan 29, 2021·Biosensors & Bioelectronics·Shazana Hilda ShamsuddinPaul A Millner
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Feb 15, 2017·Analytical Chemistry·Eleni KoutsoumpeliSteven Johnson

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Methods Mentioned

BETA
ELISA
surface plasmon resonance
sandwich immunoassay
biosensing
biosensor
biosensors
phage display

Software Mentioned

ProFit
EC
lab

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