Label-Free, Flow-Imaging Methods for Determination of Cell Concentration and Viability

Pharmaceutical Research
A S SediqW Jiskoot

Abstract

To investigate the potential of two flow imaging microscopy (FIM) techniques (Micro-Flow Imaging (MFI) and FlowCAM) to determine total cell concentration and cell viability. B-lineage acute lymphoblastic leukemia (B-ALL) cells of 2 different donors were exposed to ambient conditions. Samples were taken at different days and measured with MFI, FlowCAM, hemocytometry and automated cell counting. Dead and live cells from a fresh B-ALL cell suspension were fractionated by flow cytometry in order to derive software filters based on morphological parameters of separate cell populations with MFI and FlowCAM. The filter sets were used to assess cell viability in the measured samples. All techniques gave fairly similar cell concentration values over the whole incubation period. MFI showed to be superior with respect to precision, whereas FlowCAM provided particle images with a higher resolution. Moreover, both FIM methods were able to provide similar results for cell viability as the conventional methods (hemocytometry and automated cell counting). FIM-based methods may be advantageous over conventional cell methods for determining total cell concentration and cell viability, as FIM measures much larger sample volumes, does not require ...Continue Reading

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Citations

Dec 12, 2020·Journal of Pharmaceutical Sciences·Dhananjay JereHanns-Christian Mahler
Jul 11, 2020·Journal of Pharmaceutical Sciences·Karin H HoogendoornWim Jiskoot
Jul 19, 2021·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·A D GrabarekT Menzen

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Methods Mentioned

BETA
flow cytometry
flow
Fluorescence-activated cell sorting
FACS

Software Mentioned

MFI View System Software ( MVSS )
Visual SpreadSheet ( FlowCAM
MFI View Analysis Suite ( MVAS )
Visual SpreadSheet
FlowCAM
MVAS
GraphPad Prism

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