Label-free fluorescence dual-amplified detection of adenosine based on exonuclease III-assisted DNA cycling and hybridization chain reaction

Biosensors & Bioelectronics
Jiewei SunLei Wang

Abstract

In this work, we constructed a label-free and dual-amplified fluorescence aptasensor for sensitive analysis of adenosine based on exonuclease III (Exo III)-assisted DNA cycling and hybridization chain reaction (HCR). Firstly, we fabricated a trifunctional probe that consisting of the catalytic strand, the aptamer sequence and a streptavidin-magnetic nanobead (streptavidin-MNB). The streptavidin-MNB played a role of enrichment and separation to achieve a low background. The aptamer sequence was employed as a recognition element to bind the target adenosine, leading to the releasing of the catalytic stand. Then, the catalytic strand induced the Exo III-assisted DNA cycling reaction and produced a large amount of DNA fragments, which got a primary amplification. Subsequently, the DNA fragments acted as trigger strands to initiate HCR, forming nicked double helices with multiple G-quadruplex structures, which achieved a secondary amplification. Finally, the G-quadruplex structures bonded with the N-nethyl mesopor-phyrin IX (NMM) and yielded an enhanced fluorescence signal, realizing the label-free detection. In the proposed strategy, a small amount of adenosine can be converted to a large amount of DNA triggers, leading to a signif...Continue Reading

References

Mar 1, 1996·Hypertension·H M Siragy, J Linden
Jul 5, 2008·Nucleosides, Nucleotides & Nucleic Acids·S GiglioniD Vannoni

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Citations

Mar 19, 2020·Chembiochem : a European Journal of Chemical Biology·Karolis NorvaišaMathias O Senge
Feb 26, 2020·Biochemistry. Biokhimii︠a︡·O L Bodulev, I Yu Sakharov
May 5, 2017·Chemistry, an Asian Journal·Dik-Lung MaChung-Hang Leung
Nov 19, 2020·Journal of Analytical Methods in Chemistry·Congcong YinMin Wang
Aug 11, 2021·ACS Applied Materials & Interfaces·Hua ChaiPeng Miao

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