Label-free fluorometric assay for cytochrome c in apoptotic cells based on near infrared Ag2 S quantum dots

Analytica Chimica Acta
Meifang CaiYuezhong Xian

Abstract

As an important biomarker, cytochrome c (Cyt c) plays a crucial role in mitochondrial electron transport chain and cell apoptosis. Herein, a label-free near-infrared (NIR) Ag2S quantum dots (QDs)-based fluorescent strategy was constructed for the sensitive and selective detection of Cyt c. In this system, Cyt c was hydrolyzed by trypsin, and the resulting heme-peptide fragment exhibited peroxidase-like activity for catalytic decomposition of H2O2 into hydroxyl radical (·OH). The presence of caffeic acid in this system resulted into the formation of caffeic acid-quinone due to the strong oxidizing ability of ·OH. The production of caffeic acid-quinone led to the fluorescence quenching of Ag2S QDs through electron transfer mechanism. Based on the cascade reaction, we successfully developed a label-free approach to detect Cyt c using Ag2S QDs as nanoprobes. Under the optimized conditions, the fluorescence intensity of Ag2S QDs was linearly relative to the concentration of Cyt c over the range from 2.0 to 150 nM with a detection limit of 1.7 nM. In addition, this strategy was successfully applied for quantitative detection of Cyt c in cell lysates of H2O2 or etoposide (anticancer drug)-induced apoptotic cells, providing great poten...Continue Reading

Citations

Nov 14, 2020·Biomaterials Science·Baskaran Purushothaman, Joon Myong Song
Jun 13, 2021·Advanced Materials·Caiping DingXiaoyuan Chen
Jul 21, 2020·ACS Omega·Svetlana V RempelAndrey A Rempel

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis