Label-free high-throughput detection and content sensing of individual droplets in microfluidic systems

Lab on a Chip
Gurkan YesilozCarolyn L Ren

Abstract

This study reports a microwave-microfluidics integrated approach capable of performing droplet detection at high-throughput as well as content sensing of individual droplets without chemical or physical intrusion. The sensing system consists of a custom microwave circuitry and a spiral-shaped microwave resonator that is integrated with microfluidic chips where droplets are generated. The microwave circuitry is very cost effective by using off-the-shelf components only. It eliminates the need for bulky benchtop equipment, and provides a compact, rapid and sensitive tool compatible for Lab-on-a-Chip (LOC) platforms. To evaluate the resonator's sensing capability, it was first applied to differentiate between single-phase fluids which are aqueous solutions with different concentrations of glucose and potassium chloride respectively by measuring its reflection coefficient as a function of frequency. The minimum concentration assessed was 0.001 g ml(-1) for potassium chloride and 0.01 g ml(-1) for glucose. In the droplet detection experiments, it is demonstrated that the microwave sensor is able to detect droplets generated at as high throughput as 3.33 kHz. Around two million droplets were counted over a period of ten minutes witho...Continue Reading

References

Nov 22, 2008·Lab on a Chip·Ramakrishna SistaVamsee Pamula
Jul 21, 2009·Proceedings of the National Academy of Sciences of the United States of America·Eric BrouzesMichael L Samuels
Aug 26, 2009·Lab on a Chip·Linas MazutisAndrew D Griffiths
Jun 19, 2010·Lab on a Chip·Charles N BaroudRémi Dangla
Sep 8, 2011·Journal of Biomedical Optics·Jing Wu, Min Gu
Feb 10, 2012·Lab on a Chip·Mira T GuoDavid A Weitz
Mar 10, 2012·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Tomasz GlawdelCarolyn L Ren
Mar 5, 2013·Lab on a Chip·Jiseok LimJean-Christophe Baret
May 18, 2013·Analytical and Bioanalytical Chemistry·Nongnoot WongkaewAntje J Baeumner
Jun 12, 2013·The Analyst·Evelien W M KemnaAlbert van den Berg
Jul 31, 2013·Lab on a Chip·Muhammed S BoybayCarolyn L Ren
Jan 11, 2014·Lab on a Chip·Yuchao ChenTony Jun Huang
Apr 20, 2014·Lab on a Chip·Hongbo ZhouShuhuai Yao
Aug 27, 2014·Proceedings of the National Academy of Sciences of the United States of America·Xiaoyun DingTony Jun Huang

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Citations

May 21, 2016·Lab on a Chip·David WongCarolyn L Ren
Oct 27, 2016·The Review of Scientific Instruments·Hee-Jo Lee, Jong-Gwan Yook
Dec 21, 2016·Lab on a Chip·Muhsincan SesenAdrian Neild
Dec 29, 2016·Analytical Chemistry·Gurkan YesilozCarolyn L Ren
Jun 21, 2017·Lab on a Chip·Muhsincan SesenAdrian Neild
Jun 29, 2017·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Sifeng MaoKatsumi Uchiyama
Feb 20, 2019·Sensors·Joni KilpijärviJari Hannu
Dec 16, 2017·Lab on a Chip·Mehmet KelleciM Selim Hanay
Dec 5, 2019·Sensors·Juliana de Novais SchiantiGustavo Pamplona Rehder
Sep 29, 2018·ACS Applied Materials & Interfaces·Surath GomisShana O Kelley

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