Label-free identification and chemical characterisation of single extracellular vesicles and lipoproteins by synchronous Rayleigh and Raman scattering

Journal of Extracellular Vesicles
Agustin Enciso-MartinezCees Otto

Abstract

Extracellular vesicles (EVs) present in blood originate from cells of different origins such as red blood cells (RBCs), platelets and leukocytes. In patients with cancer, a small portion of EVs originate from tumour cells and their load is associated with poor clinical outcome. Identification of these tumour-derived extracellular vesicles (tdEVs) is difficult as they are outnumbered by EVs of different tissue of origin as well a large number of lipoproteins (LPs) that are in the same size range. In order to detect tdEVs from the abundant presence of other particles, single-particle techniques are necessary. Here, synchronous Rayleigh and Raman scattering is used for that purpose. This combination of light scattering techniques identifies optically trapped single particles based on Rayleigh scattering and distinguishes differences in chemical composition of particle populations based on Raman scattering. Here, we show that tdEVs can be distinguished from RBC EVs and LPs in a label-free manner and directly in suspension.

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Citations

Jun 26, 2020·Frontiers in Oncology·L G RikkertL W M M Terstappen
Apr 23, 2021·Advanced Drug Delivery Reviews·Alice GualerziMarzia Bedoni
May 21, 2021·Journal of Extracellular Vesicles·Yaxuan LiangMengrou Lu
Jun 18, 2021·Nature Protocols·Guillermo Bordanaba-FloritJuan M Falcón-Pérez

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Methods Mentioned

BETA
light scattering
size exclusion chromatography
elastic light scattering
Raman scattering
flow cytometry
flow-cytometry
Rayleigh scattering
Raman
PCA
electrophoresis

Software Mentioned

MATLAB
BEADS
Eigenvector
Flow

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