Label-Free Imaging of Dynamic and Transient Calcium Signaling in Single Cells

Angewandte Chemie
Jin Lu, Jinghong Li

Abstract

Cell signaling consists of diverse events that occur at various temporal and spatial scales, ranging from milliseconds to hours and from single biomolecules to cell populations. The pathway complexities require the development of new techniques that detect the overall signaling activities and are not limited to quantifying a single event. A plasmonic-based electrochemical impedance microscope (P-EIM) that can provide such data with excellent temporal and spatial resolution and does not require the addition of any labels for detection has now been developed. The highly dynamic and transient calcium signaling activities at the early stage of G-protein-coupled receptor (GPCR) stimulation were thus studied. It could be shown that a subpopulation of cells is more responsive towards agonist stimulation, and the heterogeneity of the local distributions and the transient activities of the ion channels during agonist-activated calcium flux in single HeLa cells were investigated.

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Citations

Oct 11, 2016·Faraday Discussions·Yixian WangNongjian Tao
Mar 17, 2017·Annual Review of Analytical Chemistry·Liang YuanWei Wang
Jun 14, 2018·Chemical Science·Tao LiuWei Wang
Sep 23, 2020·Nature Methods·Pengfei ZhangNongjian Tao
Apr 14, 2018·Analytical and Bioanalytical Chemistry·Yu-Wen Su, Wei Wang
Dec 8, 2020·Biomedical Optics Express·Sidahmed A Abayzeed

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