Label-free immunosensor for the detection of kanamycin using Ag@Fe₃O₄ nanoparticles and thionine mixed graphene sheet

Biosensors & Bioelectronics
Shujun YuYong Zhang

Abstract

A highly sensitive label-free immunosensor for the detection of kanamycin had been developed using silver hybridized mesoporous ferroferric oxide nanoparticles (Ag@Fe₃O₄ NPs) and thionine mixed graphene sheet (TH-GS). TH was used as an electron transfer mediator. The electrical signal was greatly improved in the presence of GS due to its good electron-transfer ability. With the advantages of large specific surface area and excellent electrical conductivity, Ag@Fe₃O₄ NPs could immobilize more antibodies of kanamycin and promote the electron transfer. Cyclic voltammetry and square wave voltammetry were used to characterize the recognition of kanamycin. The proposed immunosensor showed good performances such as low detection limit (15 pg mL⁻¹), wide linear range (from 0.050 to 16 ng mL⁻¹), short analysis time (3 min), high stability, and good selectivity in the detection of kanamycin. The immunosensor was evaluated for pork meat sample, receiving satisfactory results.

References

Jan 23, 2003·Journal of Agricultural and Food Chemistry·Elma E M G LoomansAart Van Amerongen
Aug 16, 2005·Analytical Chemistry·Danke XuZhenqiu Ma
Dec 15, 2005·Nano Letters·Rupa MukhopadhyayFlemming Besenbacher
Jul 30, 2008·Journal of the American Chemical Society·Zhuang LiuHongjie Dai
May 3, 2011·Analytical Biochemistry·Kyung-Mi SongChangill Ban
Mar 6, 2012·ACS Nano·Omid AkhavanReza Rahighi
Dec 6, 2012·Biosensors & Bioelectronics·Rui FengQin Wei

Citations

Nov 19, 2015·ACS Nano·Jitendra N TiwariKwang S Kim
Apr 15, 2016·IET Nanobiotechnology·Chandni KhuranaBhupendra Chudasama
Jan 13, 2015·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Huimin DuanChuannan Luo
Jul 12, 2016·Biosensors & Bioelectronics·Paulo A Raymundo-PereiraOsvaldo N Oliveira
Sep 11, 2016·International Journal of Biological Macromolecules·Jiao LuXiao-Kun Ouyang
Oct 31, 2014·Scientific Reports·Maria Eugênia F BrolloMarcelo Knobel
Apr 8, 2017·Sensors·Benoît Piro, Steeve Reisberg
May 23, 2017·Physical Chemistry Chemical Physics : PCCP·Ewelina KrzyszkowskaAleksandra Wojcik
Dec 4, 2014·Analytica Chimica Acta·Neeta ThapliyalRajendra N Goyal
Jul 29, 2017·Chemical Reviews·Zdeněk FarkaPetr Skládal
Mar 8, 2020·Journal of the Science of Food and Agriculture·Mustafa Oguzhan Caglayan
May 26, 2017·Nanomaterials·Cecilia CristeaRamona Galatus
Jul 24, 2020·International Journal of Environmental Research and Public Health·Shan HeDominic Rowan Organ
Apr 3, 2021·Micromachines·Norah Salem AlsaiariMohamed A Tahoon
Mar 29, 2021·Talanta·Fariba Garkani NejadIran Sheikhshoaie
May 8, 2018·Trends in Analytical Chemistry : TRAC·Hedieh MalekzadMichael R Hamblin

Related Concepts

C.I. 52000
Antibiotics
Graphene
Kantrex
Meat
Phenothiazines
Silver
Biosensing Techniques
Magnetite
Nanocrystalline Materials

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