Label-Free Impedimetric Nanoband Sensor for Detection of Both Bovine Viral Diarrhoea Virus (BVDV) and Antibody (BVDAb) in Serum

Niamh Creedonalan O'Riordan


In veterinary medicine, diagnostic tools enabling early detection of infectious disease in cattle could play a pivotal role in the control and eradication of bovine viral diarrhoea (BVD). Early identification of cattle persistently infected with bovine viral diarrhoea virus (BVDV) is critical for early diagnosis and isolation of the animal from a susceptible herd. The immediate availability of electronic test results to a veterinarian on-farm, would eliminate the need to submit samples to a commercial laboratory thereby improving animal therapeutic outcomes considerably. An impedimetric silicon chip-based biosensor platform, containing six gold nanoband electrodes as six individual sensors, was developed in this study for detection of BVD disease target molecules. Gold nanoband electrodes were first coated with an electrodeposited polymer and then modified with either monoclonal antibodies or Erns protein, for the subsequent detection of BVD virus and antibodies in whole serum, respectively. We demonstrate that the nanoband sensors have sufficient sensitivity and specificity for serological detection of both targets, with a low time-to-result (20 minutes). All serological samples were benchmarked against, and in complete agreem...Continue Reading

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