Label-free liquid crystal biosensor for L-histidine: A DNAzyme-based platform for small molecule assay

Biosensors & Bioelectronics
Shuzhen LiaoRu-Qin Yu

Abstract

We have developed a novel DNAzyme-based liquid crystal (LC) biosensor with high sensitivity for L-histidine, which is based on L-histidine-mediated formation of DNA duplexes by cleaving DNAzyme using L-histidine, resulting in a remarkable optical signal. Firstly, an optimal amount of capture probe is bound to the glass slide, which changes the surface topology as little as possible and shows a zero-background for the sensing system. When the DNAzyme molecule is cleaved by the target, L-histidine, a partial substrate strand is produced, which in turn can hybridize with the capture probe, forming a DNA duplex. The DNA duplexes induce LC molecules to undergo a homeotropic-to-tiled transition, obtaining a remarkable optical signal. The results show that the DNAzyme-based LC biosensor is highly sensitive to L-histidine with a detection limit of 50 nM. Compared with previously reported multi-step amplified methods, this newly designed assay system for L-histidine has no amplified procedures with comparable sensitivity. This method is an unprecedented example of DNAzyme-based LC biosensor for small molecules, which has potential to offer a DNAzyme-based LC model used in various targets.

References

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Citations

Sep 27, 2016·Biosensors & Bioelectronics·Mashooq Khan, Soo-Young Park
Jan 23, 2019·The Analyst·JiaYin DuXingLiang Xiong
Feb 13, 2020·Micromachines·Chonglin LuanDawei Luo
May 13, 2021·Soft Matter·Ziyihui WangTiegen Liu
Jul 14, 2021·Chemical Reviews·Min LiQiang Xia

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