Label-free luminescence switch-on detection of T4 polynucleotide kinase activity using a G-quadruplex-selective probe

Chemical Communications : Chem Comm
Hong-Zhang HeDik-Lung Ma

Abstract

A label-free, oligonucleotide-based, switch-on luminescence detection method for T4 polynucleotide kinase activity has been developed using a novel G-quadruplex-selective luminescent Ir(iii) complex probe. The application of the assay for screening potential T4 PNK inhibitors is also demonstrated. To our knowledge, this is the first metal-based assay for PNK activity.

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Citations

Oct 1, 2014·International Journal of Molecular Sciences·Václav BrázdaMiroslav Fojta
Aug 12, 2014·Expert Opinion on Drug Discovery·Lucia ParrottaAnna Artese
Nov 5, 2014·Accounts of Chemical Research·Dik-Lung MaChung-Hang Leung
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Jun 29, 2015·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Min Xu, Baoxin Li
Jul 21, 2015·Chemistry & Biology·Dik-Lung MaChung-Hang Leung
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Mar 28, 2017·Dalton Transactions : an International Journal of Inorganic Chemistry·Yuying LiuLi Xu

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