Label-Free Mass Spectrometry-Based Quantitative Proteomics Analysis of Serum Proteins During Early Pregnancy in Jennies (Equus asinus )

Frontiers in Veterinary Science
Liang DengZheng Li

Abstract

Early pregnancy in jennies is routinely determined by palpation per rectum or ultrasonography and also by detecting steroid hormone and chorionic gonadotropin levels in the blood, plasma, and serum. Herein we applied label-free mass spectrometry-based quantitative proteomics to identify serum proteins that were differentially expressed between early pregnant (day 45 after ovulation) and non-pregnant jennies. Bioinformatics analysis allowed illustration of pathways potentially involved in early pregnancy. We identified 295 proteins from a total of 2,569 peptides. Twenty-five proteins (22 upregulated and three downregulated) were significantly differentially expressed between the early pregnant and non-pregnant groups. The majority of the differentially expressed proteins were involved in defense response, early embryonic development, and hormone signaling pathways. Furthermore, functional protein analyses suggested that proteins were involved in binding, enzyme inhibitor activity, and enzyme regulator activity. Five serum proteins-granulin precursor/acrogranin, transgelin-2, fibronectin, fibrinogen-like 1, and thrombospondin 1-can be considered as novel, reliable candidates to detect pregnancy in jennies. To the best of our know...Continue Reading

References

Apr 16, 1998·The Journal of Clinical Investigation·J LawlerR O Hynes
Nov 1, 2001·Annual Review of Cell and Developmental Biology·J C Adams
Sep 24, 2002·Journal of Cell Science·Roumen Pankov, Kenneth M Yamada
Jan 14, 2003·Nature Medicine·Zhiheng HeAndrew Bateman
Jul 15, 2003·Journal of Cell Science·Iwona Wierzbicka-Patynowski, Jean E Schwarzbauer
Sep 21, 2004·Proteomics·Ingrid MillerManfred Gemeiner
May 13, 2006·Journal of Thrombosis and Haemostasis : JTH·H Ni
Sep 26, 2006·Biochemical and Biophysical Research Communications·D C RijkenF W G Leebeek
Nov 28, 2007·Biochemical and Biophysical Research Communications·Zhilin Liu, Chinweike Ukomadu
May 20, 2008·BJOG : an International Journal of Obstetrics and Gynaecology·A LarssonO Axelsson
Jun 24, 2008·The Journal of Clinical Investigation·Kai KessenbrockDieter E Jenne
Apr 21, 2009·Nature Methods·Jacek R WiśniewskiMatthias Mann
Nov 17, 2009·Journal of Biomedicine & Biotechnology·Wenhong ZhuChun-Ming Huang
Aug 31, 2011·Cold Spring Harbor Perspectives in Biology·Josephine C Adams, Jack Lawler
Sep 20, 2011·Journal of Clinical Bioinformatics·Akos VégváriGyörgy Marko-Varga
Oct 26, 2011·The British Journal of Nutrition·Andrea HenzeFlorian J Schweigert
May 2, 2012·Journal of Proteomics·Fausto ScoppettaElisabetta Chiaradia
Oct 17, 2014·Equine Veterinary Journal·M AliA Nadeem
Jan 1, 2013·Annual Review of Animal Biosciences·D F AntczakW R Allen
Dec 24, 2014·Animal Science Journal = Nihon Chikusan Gakkaihō·Enkhbolor BarsurenHong Sik Kong
May 1, 2015·Asian-Australasian Journal of Animal Sciences·Jae Eun LeeDong Il Jin
Nov 1, 2015·Methods in Molecular Biology·Emmanuel BoutetIoannis Xenarios
Jun 10, 2016·Reproduction : the Official Journal of the Society for the Study of Fertility·Denis AleksicRobert Kammerer
Jun 28, 2016·Nature Methods·Stefka TyanovaJürgen Cox
Apr 19, 2017·Reproduction, Fertility, and Development·Justyna KolakowskaAnita Franczak
Jul 12, 2017·Animal : an International Journal of Animal Bioscience·A LepczyńskiA Herosimczyk
Nov 8, 2017·Journal of Agricultural and Food Chemistry·Xueyan CaoXiqing Yue
Feb 1, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Xiaoling LiangShangrong Fan
Feb 2, 2020·Translational Research : the Journal of Laboratory and Clinical Medicine·Lin KangHung-Tsung Wu

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Methods Mentioned

BETA
electrophoresis
protein assay
acetylation

Software Mentioned

R
MaxQuant
Perseus
Cytoscape
Excel

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