Label-free microelectronic PCR quantification

Analytical Chemistry
Chih-Sheng Johnson HouScott R Manalis

Abstract

We present a robust and simple method for direct, label-free PCR product quantification using an integrated microelectronic sensor. The field-effect sensor can sequentially detect the intrinsic charge of multiple unprocessed PCR products and does not require sample processing or additional reagents in the PCR mixture. The sensor measures nucleic acid concentration in the PCR relevant range and specifically detects the PCR products over reagents such as Taq polymerase and nucleotide monomers. The sensor can monitor the product concentration at various stages of PCR and can generate a readout that resembles that of a real-time fluorescent measurement using an intercalating dye but without its potential inhibition artifacts. The device is mass-produced using standard semiconductor processes, can be reused for months, and integrates all sensing components directly on-chip. As such, our approach establishes a foundation for the direct integration of PCR-based in vitro biotechnologies with microelectronics.

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Citations

Aug 30, 2006·Proceedings of the National Academy of Sciences of the United States of America·Nebojsa M MilovicScott R Manalis
Jul 27, 2011·Biomedical Microdevices·Eric SalmRashid Bashir
Nov 1, 2008·Expert Opinion on Drug Discovery·Richard F WinkleSanjiv Sharma
Jul 16, 2013·IEEE Transactions on Biomedical Circuits and Systems· Seong-Jin Kim, Euisik Yoon
Jul 10, 2013·Biosensors & Bioelectronics·Yifan Liu, Levent Yobas
Feb 27, 2014·Scientific Reports·Antoine BlinUlrich Bockelmann
Aug 28, 2007·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Petra A NeffAndreas R Bausch
Mar 3, 2007·Lab on a Chip·Chih-Sheng Johnson HouScott R Manalis
Sep 6, 2008·Sensors·Thomas Ming-Hung Lee
Oct 8, 2020·Sensors·Arshak Poghossian, Michael J Schöning
Sep 3, 2009·Journal of the American Chemical Society·Thibaut DeféverDamien Marchal
Feb 2, 2011·Analytical Chemistry·Thibaut DeféverBenoit Limoges
May 18, 2018·Analytical Chemistry·Thomas S BronderMichael J Schöning

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