Label-free multi-photon imaging of dysplasia in Barrett's esophagus

Biomedical Optics Express
S MehravarK Kieu

Abstract

Barrett's esophagus (BE) is a metaplastic disorder where dysplastic and early cancerous changes are invisible to the naked eye and where the practice of blind biopsy is hampered by large sampling errors. Multi-photon microscopy (MPM) has emerged as an alternative solution for fast and label-free diagnostic capability for identifying the histological features with sub-micron accuracy. We developed a compact, inexpensive MPM system by using a handheld mode-locked fiber laser operating at 1560nm to study mucosal biopsies of BE. The combination of back-scattered THG, back-reflected forward THG and SHG signals generate images of cell nuclei and collagen, leading to label-free diagnosis in Barrett's.

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Citations

Apr 11, 2018·Journal of Biomedical Optics·Benjamin CromeyBhaskar Banerjee
Oct 19, 2017·Optics Express·Babak AmirsolaimaniKhanh Kieu
Aug 5, 2020·Applied Optics·Soroush MehravarKhanh Kieu
May 16, 2018·Biomedical Optics Express·Farhad AkhoundiKhanh Kieu
Jul 19, 2020·Optics Express·Masaaki Hashimoto, Yoshihiro Taguchi
Apr 3, 2021·Biomedical Optics Express·Hong ZhangXin Zhu

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