Nov 19, 2019

Label-free multiphoton imaging of β -amyloid plaques in Alzheimer's disease mouse models

Shu WangJianxin Chen


β -Amyloid ( A β ) plaque, representing the progressive accumulation of the protein that mainly consists of A β , is one of the prominent pathological hallmarks of Alzheimer's disease (AD). Label-free imaging of A β plaques holds the potential to be a histological examination tool for diagnosing AD. We applied label-free multiphoton microscopy to identify extracellular A β plaque as well as intracellular A β accumulation for the first time from AD mouse models. We showed that a two-photon-excited fluorescence signal is a sensitive optical marker for revealing the spatial-temporal progression and the surrounding morphological changes of A β deposition, which demonstrated that both extracellular and intracellular A β accumulations play an important role in the progression of AD. Moreover, combined with a custom-developed image-processing program, we established a rapid method to visualize different degrees of A β deposition by color coding. These results provide an approach for investigating pathophysiology of AD that can complement traditional biomedical procedures.

  • References
  • Citations


  • We're still populating references for this paper, please check back later.
  • References
  • Citations


  • This paper may not have been cited yet.

Mentioned in this Paper

Biological Markers
Alzheimer's Disease
Mouse Model
Beta protein
Diagnostic Imaging
Laboratory mice
Disease Progression
Microscopy, Fluorescence, Multiphoton

Related Feeds

Alzheimer's Disease: Animal models

Alzheimer's disease is a chronic neurodegenerative disease which can be studied using various experimental systems. This feed focuses on animal models used for Alzheimer's disease research.