Label-free multiphoton microscopy reveals relevant tissue changes induced by alginate hydrogel implantation in rat spinal cord injury

Scientific Reports
Roberta GalliMatthias Kirsch

Abstract

The development of therapies promoting recovery after spinal cord injury is a challenge. Alginate hydrogels offer the possibility to develop biocompatible implants with mechanical properties tailored to the nervous tissue, which could provide a permissive environment for tissue repair. Here, the effects of non-functionalized soft calcium alginate hydrogel were investigated in a rat model of thoracic spinal cord hemisection and compared to lesioned untreated controls. Open field locomotion tests were employed to evaluate functional recovery. Tissue analysis was performed with label-free multiphoton microscopy using a multimodal approach that combines coherent anti-Stokes Raman scattering to visualize axonal structures, two-photon fluorescence to visualize inflammation, second harmonic generation to visualize collagenous scarring. Treated animals recovered hindlimb function significantly better than controls. Multiphoton microscopy revealed that the implant influenced the injury-induced tissue response, leading to decreased inflammation, reduced scarring with different morphology and increased presence of axons. Demyelination of contralateral white matter near the lesion was prevented. Reduced chronic inflammation and increased a...Continue Reading

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Citations

Jun 29, 2021·Frontiers in Cardiovascular Medicine·Ishita TandonKartik Balachandran
Aug 8, 2021·European Journal of Trauma and Emergency Surgery : Official Publication of the European Trauma Society·Atefeh JahandidehFatemeh Ramezani
Mar 17, 2020·Carbohydrate Polymers·Kokkarachedu VaraprasadEmmanuel Rotimi Sadiku

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Methods Mentioned

BETA
imaging techniques
rheology

Software Mentioned

Fiji
CARS
ZEN
GraphPad Prism
GraphPad
Stereo Investigator

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