Label-free nonlinear optical imaging of mouse retina

Biomedical Optics Express
Sicong HeJianan Y Qu

Abstract

A nonlinear optical (NLO) microscopy system integrating stimulated Raman scattering (SRS), two-photon excited fluorescence (TPEF) and second-harmonic generation (SHG) was developed to image fresh mouse retinas. The morphological and functional details of various retinal layers were revealed by the endogenous NLO signals. Particularly, high resolution label-free imaging of retinal neurons and nerve fibers in the ganglion cell and nerve fiber layers was achieved by capturing endogenous SRS and TPEF signals. In addition, the spectral and temporal analysis of TPEF images allowed visualization of different fluorescent components in the retinal pigment epithelium (RPE). Fluorophores with short TPEF lifetime, such as A2E, can be differentiated from other long-lifetime components in the RPE. The NLO imaging method would provide important information for investigation of retinal ganglion cell degeneration and holds the potential to study the biochemical processes of visual cycle in the RPE.

References

May 1, 1990·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C S von BartheldE W Rubel
Jun 1, 1986·The Journal of Comparative Neurology·T Voigt
Aug 1, 1986·Mechanisms of Ageing and Development·M L KatzW G Robison
Apr 20, 2002·Biophysical Journal·Shaohui HuangWatt W Webb
Aug 15, 2002·Proceedings of the National Academy of Sciences of the United States of America·Aikaterini ZoumiBruce J Tromberg
May 21, 2003·Proceedings of the National Academy of Sciences of the United States of America·Warren R ZipfelWatt W Webb
Nov 13, 2003·Vision Research·Janet R SparrowKoji Nakanishi
Jan 28, 2004·The Journal of Cell Biology·Yoshikazu ImanishiKrzysztof Palczewski
Apr 7, 2004·Proceedings of the National Academy of Sciences of the United States of America·Roxana A RaduGabriel H Travis
Jul 28, 2004·Investigative Ophthalmology & Visual Science·Hendrik P N SchollAlan C Bird
May 3, 2005·Experimental Eye Research·Janet R Sparrow, Mike Boulton
Jul 1, 2005·Physiological Reviews·Olaf Strauss
Mar 11, 2006·Journal of Biomedical Optics·Meng HanJosef F Bille
Apr 22, 2006·Neuroscience·J CoombsL M Chalupa
Sep 28, 2006·Investigative Ophthalmology & Visual Science·Almut Bindewald-WittichFrank G Holz
Nov 10, 2006·Journal of Biomedical Optics·Yicong Wu, Jianan Y Qu
Mar 30, 2007·Microscopy Research and Technique·D SchweitzerA Bergmann
Sep 14, 2007·The Journal of Comparative Neurology·Luis Pérez De Sevilla MüllerReto Weiler
Dec 15, 2007·Journal of Neuroscience Methods·Christopher K S LeungRobert N Weinreb
Nov 22, 2008·Journal of Biomedical Optics·Dan FuWarren S Warren
Nov 22, 2008·Journal of Biomedical Optics·Wei ZhengJianan Y Qu
Jan 15, 2010·Investigative Ophthalmology & Visual Science·Florent AptelEmmanuel Beaurepaire
Feb 18, 2011·Biomedical Optics Express·Wei ZhengJianan Y Qu
Mar 31, 2011·Investigative Ophthalmology & Visual Science·Andrew W JohnsonMalik Y Kahook
Nov 30, 2011·Ophthalmic Research·Elena Prokofyeva, Eberhart Zrenner
Dec 22, 2012·The Journal of Physical Chemistry. B·Dan FuXiaoliang Sunney Xie
Feb 21, 2013·Investigative Ophthalmology & Visual Science·Katherine T JanssenRobert W Nickells
Sep 7, 2013·Biomedical Optics Express·Robin SharmaJennifer J Hunter

❮ Previous
Next ❯

Citations

Dec 15, 2016·Chemical Reviews·Richard C PrinceEric O Potma
Mar 10, 2018·Journal of Biophotonics·Sicong HeJianan Y Qu
Mar 17, 2017·Journal of Biomedical Optics·Katarina BukaraAleksandar J Krmpot
Dec 1, 2017·Biomedical Optics Express·Gabrielle A MurashovaMarcos Dantus
Oct 1, 2019·Biomedical Optics Express·Daniel J WahlMarinko V Sarunic
Oct 6, 2020·Biomedical Optics Express·Juan M BuenoNazario Bautista-Elivar
Jul 20, 2019·Alzheimer's Research & Therapy·Jurre den HaanKeir X X Yong

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.