Label-Free Proteomic Analysis of Flavohemoglobin Deleted Strain of Saccharomyces cerevisiae

International Journal of Proteomics
Chiranjit PanjaSanjay Ghosh

Abstract

Yeast flavohemoglobin, YHb, encoded by the nuclear gene YHB1, has been implicated in the nitrosative stress responses in Saccharomyces cerevisiae. It is still unclear how S. cerevisiae can withstand this NO level in the absence of flavohemoglobin. To better understand the physiological function of flavohemoglobin in yeast, in the present study a label-free differential proteomics study has been carried out in wild-type and YHB1 deleted strains of S. cerevisiae grown under fermentative conditions. From the analysis, 417 proteins in Y190 and 392 proteins in ΔYHB1 were identified with high confidence. Interestingly, among the differentially expressed identified proteins, 40 proteins were found to be downregulated whereas 41 were found to be upregulated in ΔYHB1 strain of S. cerevisiae (p value < 0.05). The differentially expressed proteins were also classified according to gene ontology (GO) terms. The most enriched and significant GO terms included nitrogen compound biosynthesis, amino acid biosynthesis, translational regulation, and protein folding. Interactions of differentially expressed proteins were generated using Search Tool for the Retrieval of Interacting Genes (STRING) database. This is the first report which offers a m...Continue Reading

References

Jun 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·H Zhu, A F Riggs
Jan 1, 1985·Critical Reviews in Microbiology·J K Bhattacharjee
Mar 24, 1995·The Journal of Biological Chemistry·M J CrawfordD E Goldberg
Jul 1, 1996·Journal of Bacteriology·M LaCelleM M Nakano
Jun 20, 1998·The Journal of Biological Chemistry·M J Crawford, D E Goldberg
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·P R GardnerA L Salzman
Nov 25, 1998·Proceedings of the National Academy of Sciences of the United States of America·A HausladenJ S Stamler
Mar 14, 2000·Natural Product Reports·T M Zabriskie, M D Jackson
Apr 12, 2000·Proceedings of the National Academy of Sciences of the United States of America·L LiuJ S Stamler
Aug 29, 2003·Biochemical and Biophysical Research Communications·Yuji SakasegawaKiyotoshi Kaneko
Sep 29, 2005·Bioinformatics·Sean D Hooper, Peer Bork
Oct 13, 2005·Molecular & Cellular Proteomics : MCP·Jeffrey C SilvaScott J Geromanos
May 20, 2006·Yeast·Susannah HoranBrigitte Meunier
Jun 17, 2006·BMC Bioinformatics·Andreas SchlickerThomas Lengauer
Jul 29, 2006·The Journal of Biological Chemistry·Iel-Soo BangFerric C Fang
Nov 30, 2006·Redox Report : Communications in Free Radical Research·Anna Lewinska, Grzegorz Bartosz
May 15, 2007·Proceedings of the National Academy of Sciences of the United States of America·Daniel R HydukeJames C Liao
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
May 26, 2009·Biochemical and Biophysical Research Communications·Rupam SahooSanjay Ghosh
Aug 12, 2009·Molecular & Cellular Proteomics : MCP·Zhuo ShenBai-Chen Wang
Aug 22, 2009·Biochemical and Biophysical Research Communications·Arindam BhattacharjeeSanjay Ghosh
Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Jiawei ZhangZheng-Zheng Shi
Feb 16, 2010·Archives of Biochemistry and Biophysics·Arindam BhattacharjeeSanjay Ghosh
Mar 24, 2011·Nature Reviews. Molecular Cell Biology·Armen ParsyanNahum Sonenberg
Jul 27, 2011·PloS One·Fran SupekTomislav Šmuc
Oct 11, 2011·Archives of Biochemistry and Biophysics·William E KarstenLilian Chooback
Nov 10, 2012·The Journal of Biological Chemistry·Meike WenkHans-Georg Koch
Dec 4, 2012·Nucleic Acids Research·Andrea FranceschiniLars J Jensen
Feb 16, 2013·Cell Death & Disease·R-F MaoZ-Z Shi
Aug 12, 2014·Biochemical and Biophysical Research Communications·Chiranjit Panja, Sanjay Ghosh

❮ Previous
Next ❯

Methods Mentioned

BETA
proteomic profiling
protein folding

Software Mentioned

PLGS
REViGO
Search Tool for the Retrieval of Interacting Genes ( STRING )
Database for Annotation , Visualization , and Integrated Disco...
ProteinLynx Global Server
CYTOSCAPE
Waters PLGS
CLONTECH
Search Tool for the Retrieval of
STRING

Related Concepts

Related Feeds

Candida albicans

Candida albicans is an opportunistic, fungal pathogen of humans that frequently causes superficial infections of oral and vaginal mucosal surfaces of debilitated and susceptible individuals. Discover the latest research on Candida albicans here.

Candidiasis

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candidiasis (ASM)

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.