Label-free proteomics uncovers energy metabolism and focal adhesion regulations responsive for endometrium receptivity

Journal of Proteome Research
Qian ChenChen Xu

Abstract

The menstrual cycle of the female uterus leads to periodic changes of the endometrium. These changes are important for developing the endometrial receptivity and for achieving competency of embryo implantation. However, the molecular events underlying the endometrial receptivity process remain poorly understood. Here we applied an LC-MS-based label-free quantitative proteomic approach to compare the endometrial tissues in the midsecretory (receptive) phase with the endometrial tissues in the proliferative phase from age-matched woman (n = 6/group). The proteomes of endometrial tissues were extracted using an SDS-based detergent, digested by the filter-aided sample preparation procedures, and subsequently analyzed by nano-LC-MS/MS (Orbitrap XL) with a 4 h gradient. Reliable protein expression profiles were reproducibly obtained from the endometrial tissues in the receptive and proliferative phases. A total of 2138 protein groups were quantified under highly stringent criteria with a false discovery rate of <1% for peptide and protein groups. Among these proteins, 317 proteins had differences in expression that were statistically significant between the receptive and proliferative phases. Direct protein-protein interaction networ...Continue Reading

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Citations

Nov 1, 2015·Molecular & Cellular Proteomics : MCP·Clement SoleilhavoupXavier Druart
Nov 1, 2018·Journal of Neurochemistry·Xiao Tian HuangHai Yan Zhang
Aug 29, 2020·Biology of Reproduction·Xi GuoXiaoyan Chen
Jan 21, 2020·Journal of Animal Science and Biotechnology·Qianying YangLei An
Feb 6, 2021·Nutrients·Anna Maria MucciniStacey J Ellery
Jun 3, 2021·International Journal of Molecular Sciences·Diqi YangYi Ding
Jul 4, 2021·Molecular Biology Reports·İrem Gülfem AlbayrakTuba Günel

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